Open Access

Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7

  • Authors:
    • Ling Wei
    • Bao‑Ming Li
    • Cheng‑Bin Wang
    • Zi‑Jia Kang
    • Jie Sun
    • Hui‑Juan Wu
    • Yong‑Zhi Lun
  • View Affiliations

  • Published online on: November 7, 2017     https://doi.org/10.3892/mmr.2017.7996
  • Pages: 870-876
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Escherichia coli (E. coli) O157:H7 is an important food‑borne pathogen with a low infective threshold and high resistance to treatment. There are currently a number of detection methods available, however, the majority are time‑consuming, complex and expensive, thus it is hard for these methods to be applied in routine detection. Therefore, there is urgent requirement to develop more sensitive, rapid and specific detective techniques. In the present study, an immuno‑biosensor based on the interference of load to the F0F1‑ATPase rotation, indicated by the fluorescence fluctuation, was constructed to detect O157:H7. The results demonstrated a good linear relationship (R2=0.9818) between antigen concentration (range, 102 cfu to 104 cfu) and the fluorescence intensity. The detection signals of the samples containing 102 cfu/well and 104 cfu/well E. coli O157:H7 were significantly stronger than the signal produced by the control sample (P<0.01). Due to its higher sensibility and simplicity when compared with the current methods applied, the results of the present study indicate a promising future for the application of this technique in detecting food source pathogens.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wei L, Li BM, Wang CB, Kang ZJ, Sun J, Wu HJ and Lun YZ: Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Mol Med Rep 17: 870-876, 2018.
APA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., & Lun, Y. (2018). Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Molecular Medicine Reports, 17, 870-876. https://doi.org/10.3892/mmr.2017.7996
MLA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17.1 (2018): 870-876.
Chicago
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17, no. 1 (2018): 870-876. https://doi.org/10.3892/mmr.2017.7996