Open Access

Identification of differentially expressed microRNAs in acute Kawasaki disease

  • Authors:
    • Ye Chen
    • Yue‑Yue Ding
    • Yan Ren
    • Lei Cao
    • Qiu‑Qin Xu
    • Ling Sun
    • Ming‑Guo Xu
    • Hai‑Tao Lv
  • View Affiliations

  • Published online on: November 8, 2017     https://doi.org/10.3892/mmr.2017.8016
  • Pages: 932-938
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in an independent cohort (n=8). Target genes of the differentially expressed miRNAs were predicted and analyzed for gene ontology term enrichment and Kyoto Encyclopedia of Genes and Genomes pathways. miRNA microarray analysis revealed that seven miRNAs (miRs) were significantly upregulated (hsa‑let‑7b‑5p, hsa‑miR‑223‑3p, hsa‑miR‑4485, hsa‑miR‑4644, hsa‑miR‑4800‑5p, hsa‑miR‑6510‑5p and hsa‑miR‑765) and three were significantly downregulated (hsa‑miR‑33b‑3p, hsa‑miR‑4443 and hsa‑miR‑4515) in acute KD compared with the healthy controls. hsa‑miR‑223‑3p expression levels detected by RT‑qPCR were consistent with the microarray results. A total of 62 target genes of hsa‑miR‑223‑3p were predicted. In total, 10 differentially expressed miRNAs were identified in acute KD, of which hsa‑miR‑223‑3p was verified by RT‑qPCR.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Chen Y, Ding YY, Ren Y, Cao L, Xu QQ, Sun L, Xu MG and Lv HT: Identification of differentially expressed microRNAs in acute Kawasaki disease. Mol Med Rep 17: 932-938, 2018.
APA
Chen, Y., Ding, Y., Ren, Y., Cao, L., Xu, Q., Sun, L. ... Lv, H. (2018). Identification of differentially expressed microRNAs in acute Kawasaki disease. Molecular Medicine Reports, 17, 932-938. https://doi.org/10.3892/mmr.2017.8016
MLA
Chen, Y., Ding, Y., Ren, Y., Cao, L., Xu, Q., Sun, L., Xu, M., Lv, H."Identification of differentially expressed microRNAs in acute Kawasaki disease". Molecular Medicine Reports 17.1 (2018): 932-938.
Chicago
Chen, Y., Ding, Y., Ren, Y., Cao, L., Xu, Q., Sun, L., Xu, M., Lv, H."Identification of differentially expressed microRNAs in acute Kawasaki disease". Molecular Medicine Reports 17, no. 1 (2018): 932-938. https://doi.org/10.3892/mmr.2017.8016