Acetylshikonin suppresses invasion of Porphyromonas gingivalis‑infected YD10B oral cancer cells by modulating the interleukin-8/matrix metalloproteinase axis

Cho,Bong‑Hae; Jung,Yun‑Hoa; Kim,Da  Jeong; Woo,Bok  Hee; Jung,Ji  Eun; Lee,Ji  Hye; Choi,Young  Whan; Park,Hae  Ryoun

doi:10.3892/mmr.2017.8151

Acetylshikonin suppresses invasion of Porphyromonas gingivalis‑infected YD10B oral cancer cells by modulating the interleukin-8/matrix metalloproteinase axis

Authors:
- Bong‑Hae Cho
- Yun‑Hoa Jung
- Da Jeong Kim
- Bok Hee Woo
- Ji Eun Jung
- Ji Hye Lee
- Young Whan Choi
- Hae Ryoun Park
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Affiliations: Department of Oral and Maxillofacial Radiology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam‑do 50612, Republic of Korea, Department of Oral Pathology and BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam‑do 50612, Republic of Korea, Department of Horticultural Bioscience, Pusan National University, Miryang, Gyeongsangnam‑do 50463, Republic of Korea
Published online on: November 24, 2017 https://doi.org/10.3892/mmr.2017.8151
Pages: 2327-2334
Copyright: © Cho et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of pharmaceutical agents possessing anti‑invasive and anti‑metastatic abilities, as well as apoptotic activity, is important in decreasing the incidence and recurrence of oral cancer. Cancer cells are known to acquire invasiveness not only through epigenetic changes, but also from inflammatory stimuli within the tumor microenvironment. Accordingly, the identification of agents that can suppress the inflammation‑promoted invasiveness of cancer cells may be important in treating cancer and improving the prognosis of patients with cancer. Acetylshikonin, a flavonoid with anti‑inflammatory activity, inhibits proliferation and induces apoptosis of oral cancer cells. In the present study, the anti‑invasive effect of acetylshikonin on YD10B oral cancer cells infected with Porphyromonas gingivalis, a major pathogen of chronic periodontitis, and the mechanisms involved were investigated. Firstly, we examined whether P. gingivalis infection increased the invasiveness of YD10B cells. Results suggested that YD10B oral cancer cells become more aggressive when they are infected with P. gingivalis. Secondly, acetylshikonin significantly inhibited the invasion of P. gingivalis‑infected YD10B cells by suppressing IL‑8 release and IL‑8‑dependent MMP release. These data suggest that acetylshikonin may be a useful preventive and therapeutic candidate for oral cancer that is chronically infected with periodontal pathogens.

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