Autophagy regulates proliferation and biliary differentiation of hepatic oval cells via the MAPK/ERK signaling pathway

  • Authors:
    • Dongdong Chen
    • Xiaoxiao Wu
    • Jie Zheng
    • Ruijie Dai
    • Zhichao Mo
    • Fahad Munir
    • Xiaolong Ni
    • Yunfeng Shan
  • View Affiliations

  • Published online on: November 27, 2017     https://doi.org/10.3892/mmr.2017.8157
  • Pages: 2565-2571
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hepatic oval cells (HOCs) are thought to possess self‑renewal ability and a bipotential capacity for differentiation, which allows them to differentiate into hepatocytes and cholangiocytes. Autophagy serves an important role in self‑renewal and differentiation of stem cells; however, how autophagy contributes to proliferation and differentiation of hepatic progenitor cells has yet to be elucidated. In the present study, autophagy was regulated by rapamycin (Rapa) and chloroquine (Chlo) administration. The results demonstrated that Chlo‑treated HOCs exhibited decreased autophagic activity alongside a decreased tendency to proliferate, as determined by Cell Counting Kit‑8. In addition, activation of autophagy by Rapa enhanced the biliary differentiation of HOCs. Furthermore, increased phosphorylated (p)‑extracellular signal‑regulated kinase (ERK)/p‑p38 expression was observed following the induction of autophagy, thus indicating that the mitogen‑activated protein kinase (MAPK)/ERK signaling pathway was activated by autophagy to exert effects on the stimulation of HOC proliferation and differentiation. In conclusion, the present study demonstrated that autophagy regulates proliferation and biliary differentiation of HOCs via the MAPK/ERK signaling pathway. These results suggest a role for autophagy in stimulating the proliferation and differentiation of HOCs.
View Figures
View References

Related Articles

Journal Cover

February-2018
Volume 17 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Wu X, Zheng J, Dai R, Mo Z, Munir F, Ni X and Shan Y: Autophagy regulates proliferation and biliary differentiation of hepatic oval cells via the MAPK/ERK signaling pathway. Mol Med Rep 17: 2565-2571, 2018.
APA
Chen, D., Wu, X., Zheng, J., Dai, R., Mo, Z., Munir, F. ... Shan, Y. (2018). Autophagy regulates proliferation and biliary differentiation of hepatic oval cells via the MAPK/ERK signaling pathway. Molecular Medicine Reports, 17, 2565-2571. https://doi.org/10.3892/mmr.2017.8157
MLA
Chen, D., Wu, X., Zheng, J., Dai, R., Mo, Z., Munir, F., Ni, X., Shan, Y."Autophagy regulates proliferation and biliary differentiation of hepatic oval cells via the MAPK/ERK signaling pathway". Molecular Medicine Reports 17.2 (2018): 2565-2571.
Chicago
Chen, D., Wu, X., Zheng, J., Dai, R., Mo, Z., Munir, F., Ni, X., Shan, Y."Autophagy regulates proliferation and biliary differentiation of hepatic oval cells via the MAPK/ERK signaling pathway". Molecular Medicine Reports 17, no. 2 (2018): 2565-2571. https://doi.org/10.3892/mmr.2017.8157