Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry

  • Authors:
    • Yi‑Lei Li
    • Rui‑Qun Qi
    • Yang Yang
    • He‑Xiao Wang
    • Hang‑Hang Jiang
    • Zheng‑Xiu Li
    • Bi‑Huan Xiao
    • Song Zheng
    • Yu‑Xiao Hong
    • Jiu‑Hong Li
    • Hong‑Duo Chen
    • Xing‑Hua Gao
  • View Affiliations

  • Published online on: November 27, 2017     https://doi.org/10.3892/mmr.2017.8159
  • Pages: 2651-2659
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Abstract

In the clinic, vitiligo is characterized by two stages: Stable and progressive. The pathogenesis of vitiligo is still not clear. Here, we identified serum markers of vitiligo by screening for differentially expressed proteins in patients with vitiligo compared to healthy individuals. Serum samples were collected from patients with vitiligo (n=10 for both the stable and progressive stages) and healthy individuals (n=10). Two‑dimensional gel electrophoresis followed by matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry and western blotting were used to validate the differential expression of the proteins in the serum (n=20 each, at both stages for patients and healthy individuals). A total of 48 differentially expressed proteins were identified by gel image analysis. There were 28 differentially expressed proteins in patients with progressive vitiligo (PV) and 13 differentially expressed proteins in patients with stable vitiligo (SV) compared with that in healthy individuals. Additionally, 7 differentially expressed proteins were identified in patients with PV compared with those in patients with SV. The western blotting results showed that Peroxiredoxin‑6, apolipoprotein L1, apolipoprotein E and mannose‑binding protein were differentially expressed in patients with different stages of vitiligo. Our results showed that change serum levels of several proteins might be useful as biomarkers or in understanding the pathogenesis of vitiligo.
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February-2018
Volume 17 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li YL, Qi RQ, Yang Y, Wang HX, Jiang HH, Li ZX, Xiao BH, Zheng S, Hong YX, Li JH, Li JH, et al: Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17: 2651-2659, 2018
APA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z. ... Gao, X. (2018). Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Molecular Medicine Reports, 17, 2651-2659. https://doi.org/10.3892/mmr.2017.8159
MLA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17.2 (2018): 2651-2659.
Chicago
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17, no. 2 (2018): 2651-2659. https://doi.org/10.3892/mmr.2017.8159