Open Access

Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells

  • Authors:
    • Zongwen Liu
    • Ting Chai
    • Yan Zhang
    • Alan Chu
    • Bing Liang
    • Xing Guo
    • Zhenjiang Guo
    • Rui Song
    • Ge Hou
    • Jinjin Yuan
    • Yaohe Liu
    • Yuhao Zhang
  • View Affiliations

  • Published online on: December 8, 2017     https://doi.org/10.3892/mmr.2017.8228
  • Pages: 2817-2826
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to detect the mutation characteristics of mitochondrial DNA (mtDNA) in Eca109 of Ec9706 cells, and to investigate their association with the nuclear genome (nDNA), thus providing a basis for gene targeting therapies for esophageal squamous cell carcinoma (ESCC). In vitro‑cultured Ec9706 and Eca109 cells were analyzed the changes of single‑nucleotide polymorphisms (SNPs), insertions/deletions (INDELs), copy number varia­tion, and structure variation (SV) of their genome by high‑throughput sequencing. The loci with SV on chromosome 1‑12 of the two ESCC cell lines were ≥5% of the mtDNA, but SV on chromosome 13‑22, X and Y was ≤3%; >40% of loci exhibited gain or loss; intergenic loci with INDEL changes and SNP features accounted for the majority of mutations. The affected genes encoded proteins including nDNA‑encoding intra‑mitochondrial‑transporting proteins, ATP energy generation‑associated proteins and mitochondrial electron respiratory chain proteins, and these proteins were all nucleus‑encoded mitochondrial proteins. The transcription, duplication, and translation of the abnormally expressed mtDNA in Ec9706 and Eca109 cells were closely associated with disorders of nuclear DNA products.
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February-2018
Volume 17 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu Z, Chai T, Zhang Y, Chu A, Liang B, Guo X, Guo Z, Song R, Hou G, Yuan J, Yuan J, et al: Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Mol Med Rep 17: 2817-2826, 2018
APA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X. ... Zhang, Y. (2018). Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Molecular Medicine Reports, 17, 2817-2826. https://doi.org/10.3892/mmr.2017.8228
MLA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17.2 (2018): 2817-2826.
Chicago
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17, no. 2 (2018): 2817-2826. https://doi.org/10.3892/mmr.2017.8228