Protective effects of psoralidin on IL‑1β‑induced chondrocyte apoptosis

Rao,Zhitao; Wang,Shuqing; Wang,Jiaqi

doi:10.3892/mmr.2017.8248

Protective effects of psoralidin on IL‑1β‑induced chondrocyte apoptosis

Authors:
- Zhitao Rao
- Shuqing Wang
- Jiaqi Wang
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Affiliations: Department of Orthopaedics, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China
Published online on: December 11, 2017 https://doi.org/10.3892/mmr.2017.8248
Pages: 3418-3424

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Abstract

Chondrocyte apoptosis serves a key role in the pathogenesis of osteoarthritis. The present study aimed to investigate the protective effects of psoralidin on interleukin (IL)‑1β‑induced chondrocyte apoptosis and explore the underlying mechanisms. Chondrocytes were isolated from the articular cartilage of Sprague‑Dawley rats and were treated with 10 ng/ml IL‑1β and various doses of psoralidin (5, 10 or 15 µM). The ratio of apoptosis was measured by Annexin V/propidium iodide double‑labeling fluorescence‑activated cell sorting (FACS) analysis. Caspase‑3 and ‑9 activity was determined using a quantitative colorimetric assay. Intracellular levels of reactive oxygen species (ROS) were assessed using a dichlorofluorescein diacetate‑labeling FACS analysis, and the release of nitric oxide (NO) was measured using the Griess reaction method. In addition, protein expression levels were detected by western blotting. The results of the present study demonstrated that psoralidin may reduce IL‑1β‑induced chondrocyte apoptosis. Psoralidin pretreatment also reversed the inhibitory effects of IL‑1β on B‑cell lymphoma 2 (Bcl‑2) expression, and decreased the IL‑1β‑induced expression of Bcl‑2‑associated X protein, matrix metalloproteinase (MMP)‑1 and MMP‑13. Furthermore, psoralidin decreased IL‑1β‑induced caspase‑3 and ‑9 activity, NO release, ROS production and nuclear factor (NF)‑κB nuclear translocation. In addition, the NF‑κB inhibitor pyrriolidine‑dithiocarbamate exerted similar effects to psoralidin, thus suggesting that IL‑1β induced proapoptotic effects in rat chondrocytes via an NF‑κB‑dependent pathway. Since psoralidin could protect chondrocytes from IL‑1β‑induced apoptosis and MMP expression, the present results suggested that psoralidin may be considered a drug candidate for the treatment of osteoarthritis.

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1	Loeser RF: Aging and osteoarthritis. Curr Opin Rheumatol. 23:492–496. 2011. View Article : Google Scholar : PubMed/NCBI
2	Rönn K, Reischl N, Gautier E and Jacobi M: Current surgical treatment of knee osteoarthritis. Arthritis. 2011:4548732011. View Article : Google Scholar : PubMed/NCBI
3	Thomas CM, Fuller CJ, Whittles CE and Sharif M: Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis Cartilage. 15:27–34. 2007. View Article : Google Scholar : PubMed/NCBI
4	Li D, Xie G and Wang W: Reactive oxygen species: The 2-edged sword of osteoarthritis. Am J Med Sci. 344:486–490. 2012. View Article : Google Scholar : PubMed/NCBI
5	Sharif M, Whitehouse A, Sharman P, Perry M and Adams M: Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3. Arthritis Rheum. 50:507–515. 2004. View Article : Google Scholar : PubMed/NCBI
6	Matsuo M, Nishida K, Yoshida A, Murakami T and Inoue H: Expression of caspase-3 and −9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage. Acta Med Okayama. 55:333–340. 2001.PubMed/NCBI
7	Koopman WJ and Moreland LW: Arthritis and allied conditions: a textbook of rheumatology. Lippincott Williams Wilkins; Philadelphia, PA: 2001
8	Koskinen A, Vuolteenaho K, Nieminen R, Moilanen T and Moilanen E: Leptin enhances MMP-1, MMP-3 and MMP-13 production in human osteoarthritic cartilage and correlates with MMP-1 and MMP-3 in synovial fluid from OA patients. Clin Exp Rheumatol. 29:57–64. 2011.PubMed/NCBI
9	Aida Y, Maeno M, Suzuki N, Shiratsuchi H, Motohashi M and Matsumura H: The effect of IL-1beta on the expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in human chondrocytes. Life Sci. 77:3210–3221. 2005. View Article : Google Scholar : PubMed/NCBI
10	Elshaier AM, Hakimiyan AA, Rappoport L, Rueger DC and Chubinskaya S: Effect of interleukin-1beta on osteogenic protein 1-induced signaling in adult human articular chondrocytes. Arthritis Rheum. 60:143–154. 2009. View Article : Google Scholar : PubMed/NCBI
11	Zhou PH, Liu SQ and Peng H: The effect of hyaluronic acid on IL-1beta-induced chondrocyte apoptosis in a rat model of osteoarthritis. J Orthop Res. 26:1643–1648. 2008. View Article : Google Scholar : PubMed/NCBI
12	Kim J, Xu M, Xo R, Mates A, Wilson GL, Pearsall AW IV and Grishko V: Mitochondrial DNA damage is involved in apoptosis caused by pro-inflammatory cytokines in human OA chondrocytes. Osteoarthritis Cartilage. 18:424–432. 2010. View Article : Google Scholar : PubMed/NCBI
13	Lee HS, Lee CH, Tsai HC and Salter DM: Inhibition of cyclooxygenase 2 expression by diallyl sulfide on joint inflammation induced by urate crystal and IL-1beta. Osteoarthritis Cartilage. 17:91–99. 2009. View Article : Google Scholar : PubMed/NCBI
14	Wuertz K, Vo N, Kletsas D and Boos N: Inflammatory and catabolic signalling in intervertebral discs: The roles of NF-κB and MAP kinases. Eur Cell Mater. 23:103–120. 2012.PubMed/NCBI
15	Radons J, Bosserhoff AK, Grässel S, Falk W and Schubert TE: p38MAPK mediates IL-1-induced down-regulation of aggrecan gene expression in human chondrocytes. Int J Mol Med. 17:661–668. 2006.PubMed/NCBI
16	Sondergaard BC, Schultz N, Madsen SH, Bay-Jensen AC and Karsdal MA: MAPKs are essential upstream signaling pathways in proteolytic degradation-divergence in pathways leading to aggrecanase and MMP mediated articular cartilage degradation. Osteoarthritis Cartilage. 18:279–288. 2010. View Article : Google Scholar : PubMed/NCBI
17	Chopra B, Dhingra AK and Dhar KL: Psoralea corylifolia L. (Buguchi)-Folklore to modern evidence: Review. Fitoterapia. 90:44–56. 2013. View Article : Google Scholar : PubMed/NCBI
18	Zhao L, Huang C, Shan Z, Xiang B and Mei L: Fingerprint analysis of Psoralea corylifolia L. by HPLC and LC-MS. J Chromatogr B Analyt Technol Biomed Life Sci. 821:67–74. 2005. View Article : Google Scholar : PubMed/NCBI
19	Wang D, Li F and Jiang Z: Osteoblastic proliferation stimulating activity of Psoralea corylifolia extracts and two of its flavonoids. Planta Med. 67:748–749. 2001. View Article : Google Scholar : PubMed/NCBI
20	Khatune NA, Islam ME, Haque ME, Khondkar P and Rahman MM: Antibacterial compounds from the seeds of Psoralea corylifolia. Fitoterapia. 75:228–230. 2004. View Article : Google Scholar : PubMed/NCBI
21	Pahari P and Rohr J: Total synthesis of psoralidin, an anticancer natural product. J Org Chem. 74:2750–2754. 2009. View Article : Google Scholar : PubMed/NCBI
22	Yu B, Wang AH, Zhou K, Chai LJ and Liu L: The molecular pathway of psoralidin-induced apoptosis in HepG2 cell line. Chin J Integr Med. Mar 29–2016.(Epub ahead of print). View Article : Google Scholar
23	Srinivasan S, Kumar R, Koduru S, Chandramouli A and Damodaran C: Inhibiting TNF-mediated signaling: A novel therapeutic paradigm for androgen independent prostate cancer. Apoptosis. 15:153–161. 2010. View Article : Google Scholar : PubMed/NCBI
24	Nakagawa S, Arai Y, Mazda O, Kishida T, Takahashi KA, Sakao K, Saito M, Honjo K, Imanishi J and Kubo T: N-acetylcysteine prevents nitric oxide-induced chondrocyte apoptosis and cartilage degeneration in an experimental model of osteoarthritis. J Orthop Res. 28:156–163. 2010.PubMed/NCBI
25	Kapoor M, Martel-Pelletier J, Lajeunesse D, Pelletier JP and Fahmi H: Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol. 7:33–42. 2011. View Article : Google Scholar : PubMed/NCBI
26	Yang YM, Hyun JW, Sung MS, Chung HS, Kim BK, Paik WH, Kang SS and Park JG: The cytotoxicity of psoralidin from Psoralea corylifolia. Planta Med. 62:353–354. 1996. View Article : Google Scholar : PubMed/NCBI
27	Mar W, Je KH and Seo EK: Cytotoxic constituents of Psoralea corylifolia. Arch Pharm Res. 24:211–213. 2001. View Article : Google Scholar : PubMed/NCBI
28	Szliszka E, Czuba ZP, Sędek Ł, Paradysz A and Król W: Enhanced TRAIL-mediated apoptosis in prostate cancer cells by the bioactive compounds neobavaisoflavone and psoralidin isolated from Psoralea corylifolia. Pharmacol Rep. 63:139–148. 2011. View Article : Google Scholar : PubMed/NCBI
29	Bronikowska J, Szliszka E, Jaworska D, Czuba ZP and Krol W: The coumarin psoralidin enhances anticancer effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Molecules. 17:6449–6464. 2012. View Article : Google Scholar : PubMed/NCBI
30	Gross A, Mcdonnell JM and Korsmeyer SJ: BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 13:1899–1911. 1999. View Article : Google Scholar : PubMed/NCBI