Open Access

Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia

  • Authors:
    • Yi Li
    • Yihao Wang
    • Hui Liu
    • Kai Ding
    • Shanfeng Hao
    • Yuanyuan Shao
    • Honglei Wang
    • Jin Chen
    • Lei Huang
    • Zonghong Shao
    • Rong Fu
  • View Affiliations

  • Published online on: December 11, 2017     https://doi.org/10.3892/mmr.2017.8252
  • Pages: 2973-2981
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interleukin (IL)-35 is the latest member of IL-12 family, which plays an important role in other autoimmune diseases. Bone marrow mononuclear cells Coombs test‑positive hemocytopenia, also termed immunorelated hemocytopenia (IRH) is a type of autoimmune-associated diseases. The present study investigated the relationship of IL‑35 in patients with IRH. A total of 43 patients with IRH and 19 normal controls were enrolled in the current study. Serum levels of IL‑35 and IL‑17 in peripheral blood were evaluated by ELISA. Regulatory T cells (Tregs) level was detected by flow cytometry and IL‑35 subunits mRNA in Treg was determined using reverse transcription‑quantitative polymerase chain reaction: Epstein‑Barr virus induced 3 (EBI3) and IL‑12α chain p35. Effect of IL‑35 on T helper 17 cells (Th17) cells was determined by mix‑culture of IL‑35 with CD4+ T lymphocytes. Serum level of IL‑35 was decreased in untreated patients with IRH compared with remission patients (P<0.01) and was significantly associated with clinical indexes. Frequency of IL‑35 produced Tregs was lower and IL‑35 subunits mRNA in CD4+CD25+ Tregs were decreased in patients with IRH compared with health controls (P<0.01). Serum level of IL‑17 was increased in patients with IRH (P<0.01) and there was a negative correlation between IL‑35 and IL‑17 (r=‑0.553; P<0.01). The production of Th17 cells and IL‑17A mRNA expression were reduced (P<0.05) after mix‑culture of CD4+ T lymphocytes with IL‑35 compared with mix‑culture of CD4+ T lymphocytes without IL‑35. In conclusion, the present study revealed that IL‑35 may be a monitoring indicator of IRH occurrence and progression. IL‑35 level was lower and the inhibition on Th17 cells was reduced in the patients with IRH.
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February-2018
Volume 17 Issue 2

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Spandidos Publications style
Li Y, Wang Y, Liu H, Ding K, Hao S, Shao Y, Wang H, Chen J, Huang L, Shao Z, Shao Z, et al: Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. Mol Med Rep 17: 2973-2981, 2018
APA
Li, Y., Wang, Y., Liu, H., Ding, K., Hao, S., Shao, Y. ... Fu, R. (2018). Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. Molecular Medicine Reports, 17, 2973-2981. https://doi.org/10.3892/mmr.2017.8252
MLA
Li, Y., Wang, Y., Liu, H., Ding, K., Hao, S., Shao, Y., Wang, H., Chen, J., Huang, L., Shao, Z., Fu, R."Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia". Molecular Medicine Reports 17.2 (2018): 2973-2981.
Chicago
Li, Y., Wang, Y., Liu, H., Ding, K., Hao, S., Shao, Y., Wang, H., Chen, J., Huang, L., Shao, Z., Fu, R."Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia". Molecular Medicine Reports 17, no. 2 (2018): 2973-2981. https://doi.org/10.3892/mmr.2017.8252