MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin Retraction in /10.3892/mmr.2023.12986

Liu,Ke; Jin,Junjun; Rong,Kunjie; Zhuo,Lukai; Li,Pingsong

doi:10.3892/mmr.2017.8264

MicroRNA‑675 inhibits cell proliferation and invasion in melanoma by directly targeting metadherin

Retraction in 10.3892/mmr.2023.12986

Authors:
- Ke Liu
- Junjun Jin
- Kunjie Rong
- Lukai Zhuo
- Pingsong Li
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Affiliations: Department of Plastic Surgery, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, P.R. China
Published online on: December 12, 2017 https://doi.org/10.3892/mmr.2017.8264
Pages: 3372-3379

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Abstract

Melanoma is derived from melanocytes and accounts for ~80% of skin cancer-associated fatalities worldwide. The dysregulation of microRNAs (miRNAs/miRs) is involved in the development and progression of melanoma. Therefore, miRNAs may be novel diagnostic or prognostic biomarkers and promising therapeutic targets in the treatment of patients with melanoma. miR‑675 is differentially expressed in several types of human cancer and has important roles in the pathogenesis of several diseases. However, the expression levels and the biological roles of miR‑675 in melanoma remain unclear. Therefore, the present study aimed to assess the expression of miR‑675 in melanoma, explore the effects of miR‑675 on melanoma cells and investigate the underlying molecular mechanisms that may be involved in the actions of miR‑675. The present study indicated that miR‑675 expression was downregulated in melanoma tissues and cell lines. Functional assays demonstrated that the upregulation of miR‑675 impaired cell proliferation and invasion in melanoma. Bioinformatics analysis, luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction and western blot analysis demonstrated that metadherin (MTDH) was a direct target of miR‑675 in melanoma. The MTDH levels were upregulated in melanoma tissues and inversely correlated with the miR‑675 expression. Furthermore, restored MTDH expression rescued the inhibition effects in melanoma cells caused by miR‑675 overexpression. Thus, miR‑675 may be a potential therapeutic target for melanoma.

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1	Miller AJ and Mihm MC Jr: Melanoma. N Engl J Med. 355:51–65. 2006. View Article : Google Scholar : PubMed/NCBI
2	Jemal A, Siegel R, Ward E, Hao Y, Xu J and Thun MJ: Cancer statistics, 2009. CA Cancer J Clin. 59:225–249. 2009. View Article : Google Scholar : PubMed/NCBI
3	Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI
4	Eggermont AM, Suciu S, Rutkowski P, Kruit WH, Punt CJ, Dummer R, Salès F, Keilholz U, de Schaetzen G and Testori A; EORTC Melanoma Group, : Long term follow up of the EORTC 18952 trial of adjuvant therapy in resected stage IIB-III cutaneous melanoma patients comparing intermediate doses of interferon-alpha-2b (IFN) with observation: Ulceration of primary is key determinant for IFN-sensitivity. Eur J Cancer. 55:111–121. 2016. View Article : Google Scholar : PubMed/NCBI
5	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI
6	Pacheco I, Buzea C and Tron V: Towards new therapeutic approaches for malignant melanoma. Expert Rev Mol Med. 13:e332011. View Article : Google Scholar : PubMed/NCBI
7	Fang W, Fan Y, Fa Z, Xu J, Yu H, Li P and Gu J: microRNA-625 inhibits tumorigenicity by suppressing proliferation, migration and invasion in malignant melanoma. Oncotarget. 8:13253–13263. 2017.PubMed/NCBI
8	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
9	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
10	Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–103. 2007. View Article : Google Scholar : PubMed/NCBI
11	Adams BD, Parsons C, Walker L, Zhang WC and Slack FJ: Targeting noncoding RNAs in disease. J Clin Invest. 127:761–771. 2017. View Article : Google Scholar : PubMed/NCBI
12	Zhang B, Pan X, Cobb GP and Anderson TA: microRNAs as oncogenes and tumor suppressors. Dev Biol. 302:1–12. 2007. View Article : Google Scholar : PubMed/NCBI
13	Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI
14	Li S, Xu X, Xu X, Hu Z, Wu J, Zhu Y, Chen H, Mao Y, Lin Y, Luo J, et al: MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos. Biochem Biophys Res Commun. 441:976–981. 2013. View Article : Google Scholar : PubMed/NCBI
15	Zhang J, Wu W, Xu S, Zhang J, Zhang J, Yu Q, Jiao Y, Wang Y, Lu A, You Y, et al: MicroRNA-105 inhibits human glioma cell malignancy by directly targeting SUZ12. Tumour Biol. 39:10104283177057662017.PubMed/NCBI
16	Asahchop EL, Akinwumi SM, Branton WG, Fujiwara E, Gill MJ and Power C: Plasma microRNA profiling predicts HIV-associated neurocognitive disorder. AIDS. 30:2021–2031. 2016. View Article : Google Scholar : PubMed/NCBI
17	Wang W, Zhang H, Tang M, Liu L, Zhou Z, Zhang S and Wang L: MicroRNA-592 targets IGF-1R to suppress cellular proliferation, migration and invasion in hepatocellular carcinoma. Oncol Lett. 13:3522–3528. 2017.PubMed/NCBI
18	Zhang P, Kong F, Deng X, Yu Y, Hou C, Liang T and Zhu L: MicroRNA-326 suppresses the proliferation, migration and invasion of cervical cancer cells by targeting ELK1. Oncol Lett. 13:2949–2956. 2017.PubMed/NCBI
19	Liang Z, Wang X, Xu X, Xie B, Ji A, Meng S, Li S, Zhu Y, Wu J, Hu Z, et al: MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway. Mol Cancer. 16:962017. View Article : Google Scholar : PubMed/NCBI
20	Zhai LL, Wang P, Zhou LY, Yin JY, Tang Q, Zhang TJ, Wang YX, Yang DQ, Lin J and Deng ZQ: Over-expression of miR-675 in formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer patients. Int J Clin Exp Med. 8:11195–11201. 2015.PubMed/NCBI
21	Vennin C, Spruyt N, Dahmani F, Julien S, Bertucci F, Finetti P, Chassat T, Bourette RP, Le Bourhis X and Adriaenssens E: H19 non coding RNA-derived miR-675 enhances tumorigenesis and metastasis of breast cancer cells by downregulating c-Cbl and Cbl-b. Oncotarget. 6:29209–29223. 2015. View Article : Google Scholar : PubMed/NCBI
22	Liu C, Chen Z, Fang J, Xu A, Zhang W and Wang Z: H19-derived miR-675contributes to bladder cancer cell proliferation by regulating p53 activation. Tumour Biol. 37:263–270. 2016. View Article : Google Scholar : PubMed/NCBI
23	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
24	Kang DC, Su ZZ, Sarkar D, Emdad L, Volsky DJ and Fisher PB: Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1. Gene. 353:8–15. 2005. View Article : Google Scholar : PubMed/NCBI
25	Huang Y and Li LP: Progress of cancer research on astrocyte elevated gene-1/Metadherin (Review). Oncol Lett. 8:493–501. 2014.PubMed/NCBI
26	Zhang Y, Peng G, Wang Y, Cui L, Wu W, Wang L, Liu C and Han X: Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis. Clin Exp Pharmacol Physiol. 44:815–826. 2017. View Article : Google Scholar : PubMed/NCBI
27	Bustos MA, Ono S, Marzese DM, Oyama T, Iida Y, Cheung G, Nelson N, Hsu SC, Yu Q and Hoon DSB: miR-200a regulates CDK4/6 Inhibitor effect by targeting CDK6 in metastatic melanoma. J Invest Dermatol. 137:1955–1964. 2017. View Article : Google Scholar : PubMed/NCBI
28	He J, Tian N, Yang Y, Jin L, Feng X, Hua J, Lin S, Wang B, Li H and Wang J: miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma. Oncol Lett. 13:2442–2448. 2017.PubMed/NCBI
29	Zeng HF, Yan S and Wu SF: MicroRNA-153-3p suppress cell proliferation and invasion by targeting SNAI1 in melanoma. Biochem Biophys Res Commun. 487:140–145. 2017. View Article : Google Scholar : PubMed/NCBI
30	Guan GF, Zhang DJ, Wen LJ, Xin D, Liu Y, Yu DJ, Su K, Zhu L, Guo YY and Wang K: Overexpression of lncRNA H19/miR-675 promotes tumorigenesis in head and neck squamous cell carcinoma. Int J Med Sci. 13:914–922. 2016. View Article : Google Scholar : PubMed/NCBI
31	Yu YQ, Weng J, Li SQ, Li B and Lv J: miR-675 promotes the growth of hepatocellular carcinoma cells through the Cdc25A pathway. Asian Pac J Cancer Prev. 17:3881–3885. 2016.PubMed/NCBI
32	Lv J, Ma L, Chen XL, Huang XH and Wang Q: Downregulation of LncRNAH19 and miR-675 promotes migration and invasion of human hepatocellular carcinoma cells through AKT/GSK-3β/Cdc25A signaling pathway. J Huazhong Univ Sci Technolog Med Sci. 34:363–369. 2014. View Article : Google Scholar : PubMed/NCBI
33	He D, Wang J, Zhang C, Shan B, Deng X, Li B, Zhou Y, Chen W, Hong J, Gao Y, et al: Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer. Mol Cancer. 14:732015. View Article : Google Scholar : PubMed/NCBI
34	Wang J, Zhang Y, Wei H, Zhang X, Wu Y, Gong A, Xia Y, Wang W and Xu M: The mir-675-5p regulates the progression and development of pancreatic cancer via the UBQLN1-ZEB1-mir200 axis. Oncotarget. 8:24978–24987. 2017.PubMed/NCBI
35	Schmitz KJ, Helwig J, Bertram S, Sheu SY, Suttorp AC, Seggewiss J, Willscher E, Walz MK, Worm K and Schmid KW: Differential expression of microRNA-675, microRNA-139-3p and microRNA-335 in benign and malignant adrenocortical tumours. J Clin Pathol. 64:529–535. 2011. View Article : Google Scholar : PubMed/NCBI
36	Zhu M, Chen Q, Liu X, Sun Q, Zhao X, Deng R, Wang Y, Huang J, Xu M, Yan J and Yu J: lncRNA H19/miR-675 axis represses prostate cancer metastasis by targeting TGFBI. FEBS J. 281:3766–3775. 2014. View Article : Google Scholar : PubMed/NCBI
37	Li C, Lei B, Huang S, Zheng M, Liu Z, Li Z and Deng Y: H19 derived microRNA-675 regulates cell proliferation and migration through CDK6 in glioma. Am J Transl Res. 7:1747–1764. 2015.PubMed/NCBI
38	Yao J, Zhang P, Li J and Xu W: MicroRNA-215 acts as a tumor suppressor in breast cancer by targeting AKT serine/threonine kinase 1. Oncol Lett. 14:1097–1104. 2017.PubMed/NCBI
39	Su ZZ, Kang DC, Chen Y, Pekarskaya O, Chao W, Volsky DJ and Fisher PB: Identification and cloning of human astrocyte genes displaying elevated expression after infection with HIV-1 or exposure to HIV-1 envelope glycoprotein by rapid subtraction hybridization, RaSH. Oncogene. 21:3592–3602. 2002. View Article : Google Scholar : PubMed/NCBI
40	Gnosa S, Shen YM, Wang CJ, Zhang H, Stratmann J, Arbman G and Sun XF: Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines. J Transl Med. 10:1092012. View Article : Google Scholar : PubMed/NCBI
41	Li J, Zhang N, Song LB, Liao WT, Jiang LL, Gong LY, Wu J, Yuan J, Zhang HZ, Zeng MS and Li M: Astrocyte elevated gene-1 is a novel prognostic marker for breast cancer progression and overall patient survival. Clin Cancer Res. 14:3319–3326. 2008. View Article : Google Scholar : PubMed/NCBI
42	Yu JQ, Zhou Q, Zhu H, Zheng FY and Chen ZW: Overexpression of astrocyte elevated gene-1 (AEG-1) in cervical cancer and its correlation with angiogenesis. Asian Pac J Cancer Prev. 16:2277–2281. 2015. View Article : Google Scholar : PubMed/NCBI
43	Dong L, Qin S, Li Y, Zhao L, Dong S, Wang Y, Zhang C and Han S: High expression of astrocyte elevated gene-1 is associated with clinical staging, metastasis and unfavorable prognosis in gastric carcinoma. Tumour Biol. 36:2169–2178. 2015. View Article : Google Scholar : PubMed/NCBI
44	Yang G, Zhang L, Lin S, Li L, Liu M, Chen H, Cao M, Liu D, Huang YR and Bo J: AEG-1 is associated with tumor progression in nonmuscle-invasive bladder cancer. Med Oncol. 31:9862014. View Article : Google Scholar : PubMed/NCBI
45	Hu G, Wei Y and Kang Y: The multifaceted role of MTDH/AEG-1 in cancer progression. Clin Cancer Res. 15:5615–5620. 2009. View Article : Google Scholar : PubMed/NCBI
46	Emdad L, Lee SG, Su ZZ, Jeon HY, Boukerche H, Sarkar D and Fisher PB: Astrocyte elevated gene-1 (AEG-1) functions as an oncogene and regulates angiogenesis. Proc Natl Acad Sci USA. 106:pp. 21300–21305. 2009; View Article : Google Scholar : PubMed/NCBI
47	Du Y, Jiang B, Song S, Pei G, Ni X, Wu J, Wang S, Wang Z and Yu J: Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. Int J Oncol. 51:63–74. 2017. View Article : Google Scholar : PubMed/NCBI
48	Zou B and Wang S: Expression and prognostic value of MTDH, Bcl-2 and E-cadherin in sinonasal malignant mucosal melanoma. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 50:1009–1014. 2015.(In Chinese). PubMed/NCBI