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Downregulation of microRNA-9 increases matrix metalloproteinase-13 expression levels and facilitates osteoarthritis onset

  • Authors:
    • Hongxin Zhang
    • Bo Song
    • Zhaoxun Pan

  • View Affiliations

  • Published online on: December 22, 2017     https://doi.org/10.3892/mmr.2017.8340
  • Pages: 3708-3714

  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Matrix metalloproteinase-13 (MMP-13) degrades collagen and other matrix components, thus playing a critical role in the development of osteoarthritis (OA). The expression level of microRNA‑9 (miR‑9) is significantly depressed in cartilage tissues of OA patients. Furthermore, bioinformatics analysis demonstrated complementary binding sites between miR‑9 and MMP‑13. The current study, therefore, investigated whether miR‑9 is involved in regulating MMP‑13 expression levels and OA onset. Cartilage tissues from OA patients and healthy individuals were compared for miR‑9, MMP‑13 and collagen type II α1 chain (Col2A1) expression levels. A dual luciferase gene reporter assay was performed to evaluate the association between miR‑9 and MMP‑13. Sodium iodoacetate was injected into the knee joint cartilage tissues to generate the rat OA model. The expression levels of miR‑9, MMP‑13 and Col2A1 were compared between the model and control rats. In addition, the OA model rats received miR‑9 agomir for further expressional assay. Cartilage tissue samples from the OA patients exhibited significantly lower miR‑9 and Col2A1 expression levels when compared with the control rats, whilst the expression level of MMP‑13 was upregulated. As the target gene of miR‑9, MMP‑13 is under the targeted regulation of miR‑9. The injection of miR‑9 agomir into the knee joint cavity significantly depressed MMP‑13 expression in the cartilage tissues of OA rats, with reduced collagen degradation and enhanced COL2A1. OA cartilage tissues have lower miR‑9 expression and higher MMP‑13 expression levels. Thus, miR‑9 inhibits the expression level of MMP‑13, decreases its inhibitory effects on COL2A1, and therefore contributes to antagonizing OA.
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March-2018
Volume 17 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Zhang H, Song B and Pan Z: Downregulation of microRNA-9 increases matrix metalloproteinase-13 expression levels and facilitates osteoarthritis onset. Mol Med Rep 17: 3708-3714, 2018.
APA
Zhang, H., Song, B., & Pan, Z. (2018). Downregulation of microRNA-9 increases matrix metalloproteinase-13 expression levels and facilitates osteoarthritis onset. Molecular Medicine Reports, 17, 3708-3714. https://doi.org/10.3892/mmr.2017.8340
MLA
Zhang, H., Song, B., Pan, Z."Downregulation of microRNA-9 increases matrix metalloproteinase-13 expression levels and facilitates osteoarthritis onset". Molecular Medicine Reports 17.3 (2018): 3708-3714.
Chicago
Zhang, H., Song, B., Pan, Z."Downregulation of microRNA-9 increases matrix metalloproteinase-13 expression levels and facilitates osteoarthritis onset". Molecular Medicine Reports 17, no. 3 (2018): 3708-3714. https://doi.org/10.3892/mmr.2017.8340