Leptin, leptin receptors and hypoxia-induced factor-1α expression in the placental bed of patients with and without preeclampsia during pregnancy

Park,Min‑Jung; Lee,Dong‑Hyung; Joo,Bo‑Sun; Lee,Young‑Joo; Joo,Jong‑Kil; An,Beum‑Soo; Kim,Seung‑Chul; Lee,Kyu‑Sup

doi:10.3892/mmr.2018.8539

Leptin, leptin receptors and hypoxia-induced factor-1α expression in the placental bed of patients with and without preeclampsia during pregnancy

Authors:
- Min‑Jung Park
- Dong‑Hyung Lee
- Bo‑Sun Joo
- Young‑Joo Lee
- Jong‑Kil Joo
- Beum‑Soo An
- Seung‑Chul Kim
- Kyu‑Sup Lee
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Affiliations: The Korea Institute for Public Sperm Bank, Busan 49241, Republic of Korea, Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Biomedical Research Institute, Busan 49241, Republic of Korea, Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang, Gyeongsangnam‑do 50463, Republic of Korea
Published online on: February 1, 2018 https://doi.org/10.3892/mmr.2018.8539
Pages: 5292-5299

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Abstract

The mechanism underlying the pathogenesis of preeclampsia (PE) has been previously investigated but remains to be elucidated. Among numerous biomarkers that are associated with the pathogenesis of PE, leptin is most frequently investigated. Although studies concerning the association between PE and the expression of leptin in the serum and placenta have been conducted, the results are conflicting and inconsistent. Furthermore, the expression of leptin and its receptors in the placental bed and their association with PE, to the best of our knowledge, has not been previously reported. Therefore, to determine the association between the expression of leptin and its receptor, and pathogenesis and onset period of PE, placental bed tissues were obtained from cesarean section deliveries. The mRNA and protein expression levels of leptin and its receptor were investigated in normal pregnancies (n=18), pregnancies complicated with early‑onset PE (n=9) and late‑onset PE (n=9) by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. The results demonstrated that the mRNA and protein expression of leptin in the placental bed was significantly increased in the PE groups compared with normal controls and was associated with the onset period of PE. Furthermore, as evidenced by immunostaining, leptin was upregulated in endothelial cells of the placental bed in the PE groups, with a particularly strong upregulation in activated endothelial cells from patients with early‑onset PE. The results of the present study indicate that altered expression of leptin in the placental bed may contribute to the pathogenesis of PE.

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