Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma

  • Authors:
    • Shuyang Hu
    • Qing Ouyang
    • Qingbao Cheng
    • Jinghan Wang
    • Feiling Feng
    • Liang Qiao
    • Wei Gan
    • Yang Shi
    • Demin Wu
    • Xiaoqing Jiang
  • View Affiliations

  • Published online on: February 8, 2018     https://doi.org/10.3892/mmr.2018.8573
  • Pages: 6028-6032
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Abstract

Cholangiocarcinoma (CCA) is an aggressive malignant tumor and the prognosis of patients with advanced stage disease remains poor. Therefore, the identification of novel treatment agents for CCA is required. In the present study, the biological effects of the diabetes therapeutic agent, phenformin, in CCA cell lines was investigated. Cell Counting Kit‑8 cell viability, cellular clone formation and subcutaneous tumor formation assays were performed, which revealed that phenformin inhibited CCA cell proliferation and growth both in vitro and in vivo. In addition, phenformin induced CCA cell apoptosis and autophagy. Phenformin partly activated the liver kinase B1 (LKB1)/5' AMP‑activated protein kinase signaling pathway to exert its biological effects on CCA cell lines, as demonstrated by knockdown of LKB1, which reversed these effects. In conclusion, the present study demonstrated the biological effects of phenformin in CCA and suggested that phenformin may be a potential novel agent for CCA treatment.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hu S, Ouyang Q, Cheng Q, Wang J, Feng F, Qiao L, Gan W, Shi Y, Wu D, Jiang X, Jiang X, et al: Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma. Mol Med Rep 17: 6028-6032, 2018.
APA
Hu, S., Ouyang, Q., Cheng, Q., Wang, J., Feng, F., Qiao, L. ... Jiang, X. (2018). Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma. Molecular Medicine Reports, 17, 6028-6032. https://doi.org/10.3892/mmr.2018.8573
MLA
Hu, S., Ouyang, Q., Cheng, Q., Wang, J., Feng, F., Qiao, L., Gan, W., Shi, Y., Wu, D., Jiang, X."Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma". Molecular Medicine Reports 17.4 (2018): 6028-6032.
Chicago
Hu, S., Ouyang, Q., Cheng, Q., Wang, J., Feng, F., Qiao, L., Gan, W., Shi, Y., Wu, D., Jiang, X."Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma". Molecular Medicine Reports 17, no. 4 (2018): 6028-6032. https://doi.org/10.3892/mmr.2018.8573