Effect of Notoginsenoside R1 on autologous adipose graft in rats

Chen,Guizong; Li,Qin; Luo,Yanping; Liu,Tao; Zhou,Shaolong; Pan,Er; Peng,Lixia

doi:10.3892/mmr.2018.8596

Effect of Notoginsenoside R1 on autologous adipose graft in rats

Authors:
- Guizong Chen
- Qin Li
- Yanping Luo
- Tao Liu
- Shaolong Zhou
- Er Pan
- Lixia Peng
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Affiliations: Department of Plastic Surgery, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Plastic Surgery, Guangzhou Mylike Aesthetic Surgery Hospital, Guangzhou, Guangdong 510000, P.R. China, Department of Plastic Surgery, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510000, P.R. China
Published online on: February 13, 2018 https://doi.org/10.3892/mmr.2018.8596
Pages: 5928-5933

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Abstract

Autologous fat particle transplantation has been widely used by surgeons. The present study evaluated the effect of Notoginsenoside R1 (NR1) treatment on rat autologous fat graft, along with the quality and retention rates. Male Sprague‑Dawley rats (n=60) received fat particle auto‑transplantation from the left abdominal cavity into lateral dorsum. A total of 14 days after surgery, NR1 in different doses (50, 100 and 200 mg/kg/day) was injected into rats, following which blood and fat graft samples were harvested at days 7, 14 and 28. Assessments were carried out by hematoxylin and eosin staining, western blotting, ELISA and immunohistochemistry (IHC). The survival rate of fat grafts was increased in three experimental groups, as detected by weight measurement. Histological scoring demonstrated that there were significant differences in tissue integrity between the 100 mg/kg/day group and the other 3 groups. hepatocyte growth factor, vascular endothelial growth factor, fibroblast growth factor, angiotensin and S100 levels in the 100 mg/kg/day NR1 group was increased compared with the other 2 treatment groups; however, all 3 treatment groups demonstrated increased expression of these proteins compared with the control group. Additionally, cluster of differentiation (CD)68 exhibited negative expression and CD31 showed weakly positive expression in all three experiments, as assessed by IHC. In conclusion, 100 mg/kg/day NR1 may potentially promote the retention rate and enhance the quality of autologous fat grafts via increasing vascularity in the recipient site. These results implicate NR1 as a therapeutic strategy for the improvement of outcome following fat graft surgery.

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