Open Access

The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells

  • Authors:
    • Jiangzhou Peng
    • Zigang Yu
    • Lei Xue
    • Jiabin Wang
    • Jun Li
    • Degang Liu
    • Qiang Yang
    • Yihui Lin
  • View Affiliations

  • Published online on: February 13, 2018     https://doi.org/10.3892/mmr.2018.8606
  • Pages: 5860-5868
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the novel mechanisms of forkhead box protein P3 (foxp3) in T regulatory (Treg) cells in lung cancer behavior. Treg cells were isolated from the peripheral blood of healthy volunteers and then co‑cultured with 95D cells. A plasmid overexpressing foxp3 was constructed and transfected into Treg cells and an MTS assay was performed to assess cell viability. Flow cytometry was performed to evaluate cell apoptosis and reverse transcription‑quantitative polymerase chain reaction was used to measure mRNA expression. A Transwell assay was used to assess cell invasion. Treg cells were successfully isolated from peripheral blood with purity of 94.26%. Foxp3 expression in Treg cells was significantly increased following co‑culture with 95D cells, while matrix metalloproteinase‑9 expression was upregulated in 95D cells co‑cultured with Treg cells. The apoptosis, invasion and migration abilities of 95D cells were suppressed by co‑culture with Treg cells, whereas the adhesive ability was enhanced. Foxp3 overexpression in Treg cells enhanced the viability and invasiveness of 95D cells, whereas cell adhesion and migration were decreased. The results of the present study demonstrate that the viability and invasiveness of 95D cells are enhanced by foxp3 overexpression in Treg cells, indicating that increased levels of foxp3 in the tumor microenvironment may promote tumor cell growth.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Peng J, Yu Z, Xue L, Wang J, Li J, Liu D, Yang Q and Lin Y: The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells. Mol Med Rep 17: 5860-5868, 2018.
APA
Peng, J., Yu, Z., Xue, L., Wang, J., Li, J., Liu, D. ... Lin, Y. (2018). The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells. Molecular Medicine Reports, 17, 5860-5868. https://doi.org/10.3892/mmr.2018.8606
MLA
Peng, J., Yu, Z., Xue, L., Wang, J., Li, J., Liu, D., Yang, Q., Lin, Y."The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells". Molecular Medicine Reports 17.4 (2018): 5860-5868.
Chicago
Peng, J., Yu, Z., Xue, L., Wang, J., Li, J., Liu, D., Yang, Q., Lin, Y."The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells". Molecular Medicine Reports 17, no. 4 (2018): 5860-5868. https://doi.org/10.3892/mmr.2018.8606