Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism 
in HepG2 human hepatoblastoma cells

Zheng,Yingjuan; Zhao,Chao; Zhang,Naijian; Kang,Wenqin; Lu,Rongrong; Wu,Huadong; Geng,Yingxue; Zhao,Yaping; Xu,Xiaoyan

doi:10.3892/mmr.2018.8633

Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells

Authors:
- Yingjuan Zheng
- Chao Zhao
- Naijian Zhang
- Wenqin Kang
- Rongrong Lu
- Huadong Wu
- Yingxue Geng
- Yaping Zhao
- Xiaoyan Xu
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Affiliations: Department of Pediatrics, Shu Yang Union Medical College Hospital, Suqian, Jiangsu 223600, P.R. China, Department of Clinical Laboratory, The 82nd Hospital of Chinese People's Liberation Army, Huaian, Jiangsu 223001, P.R. China, Department of Postgraduates, BengBu Medical College, Bengbu, Anhui 233004, P.R. China
Published online on: February 26, 2018 https://doi.org/10.3892/mmr.2018.8633
Pages: 5635-5641
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the effects of hyperthyroidism on the serum levels of the microRNA (miR) miR‑206 and the role of miR‑206 on TH‑regulated lipid metabolism in liver cells. Serum was obtained from 12 patients diagnosed with hyperthyroidism and 10 healthy control subjects. Human hepatoblastoma (HepG2) cells were used to study the effects of triiodothyronine (T3) and miR‑206 on lipid metabolism. Expression of miR‑206 in serum and cells was determined by reverse transcription‑quantitative polymerase chain reaction analysis. Lipid accumulation in HepG2 cells was assessed with Oil Red O staining. Suppression or overexpression of miR‑206 was performed via transfection with a miR‑206 mimic or miR‑206 inhibitor. Serum miR‑206 was significantly decreased in patients with hyperthyroidism compared with euthyroid controls. Treatment of HepG2 cells with T3 led to reduced total cholesterol (TC) and triglyceride (TG) content, accompanied by reduced miR‑206 expression. Inhibition of endogenous miR‑206 expression decreased intracellular TG and TC content in HepG2 cells. By contrast, overexpression of miR‑206 in HepG2 partially prevented the reduction in TG content induced by treatment with T3. In conclusion, serum miR‑206 expression is reduced in patients with hyperthyroidism. In addition, miR‑206 is involved in T3‑mediated regulation of lipid metabolism in HepG2 cells, indicating a role for miR‑206 in thyroid hormone‑induced disorders of lipid metabolism in the liver.

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