Open Access

Detection of epitopes in systemic lupus erythematosus using peptide microarray

  • Authors:
    • Xin Li
    • Haixia Li
    • Qiongdan Hu
    • Jinfei Lin
    • Qiong Zhang
    • Yao Li
    • Juan Li
    • Tao Chen
    • Yurong Qiu
  • View Affiliations

  • Published online on: March 9, 2018     https://doi.org/10.3892/mmr.2018.8710
  • Pages: 6533-6541
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Systemic lupus erythematosus (SLE) is a common autoimmune disease, which features the secretion of antibodies directed against autoantigens in vivo. In the present study, a peptide microarray was developed to detect the epitopes recognized by autoantibodies in patients with SLE for an effective method of diagnosis. SLE‑associated epitopes in 14 autoantigens were predicted using the antigenic epitope prediction software DNA star. Peptides were synthesized based on the predicted antigenic epitopes and immobilized on a slide surface and developed into a peptide microarray. Using this peptide microarray the autoantibodies in 120 patients with SLE and 110 healthy subjects were detected. A total of 73 potential antigenic epitopes in 14 autoantigens were predicted and screened. The peptide microarray based on the 73 epitopes was used to detect the autoantibodies in patients with SLE. A total of 14 epitopes with potential diagnostic values were screened out. The sensitivity and specificity of the 14 epitopes for the diagnosis of SLE were 71.6 and 85.8%, respectively. An optimal set of epitopes for SLE diagnosis was obtained. As individual patients had a specific autoantibody spectrum it was possible to detect autoantibodies in SLE and perform the diagnosis of SLE using the peptide microarray.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Li H, Hu Q, Lin J, Zhang Q, Li Y, Li J, Chen T and Qiu Y: Detection of epitopes in systemic lupus erythematosus using peptide microarray. Mol Med Rep 17: 6533-6541, 2018
APA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y. ... Qiu, Y. (2018). Detection of epitopes in systemic lupus erythematosus using peptide microarray. Molecular Medicine Reports, 17, 6533-6541. https://doi.org/10.3892/mmr.2018.8710
MLA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17.5 (2018): 6533-6541.
Chicago
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17, no. 5 (2018): 6533-6541. https://doi.org/10.3892/mmr.2018.8710