Open Access

Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis

  • Authors:
    • Weiqing Qian
    • Yan Su
    • Yajie Zhang
    • Nianwei Yao
    • Nin Gu
    • Xu Zhang
    • Hong Yin
  • View Affiliations

  • Published online on: March 9, 2018     https://doi.org/10.3892/mmr.2018.8715
  • Pages: 6515-6525
  • Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteoporosis is a serious public health problem and icariin (ICA) is the active component of the Epimedium sagittatum, a traditional Chinese medicinal herb. The present study aimed to investigate the effects and underlying mechanisms of ICA as a potential therapy for osteoporosis. Calvaria osteoblasts were isolated from newborn rats and treated with ICA. Cell viability, apoptosis, alkaline phosphatase activity and calcium deposition were analyzed. Bioinformatics analyses were performed to identify differentially expressed proteins (DEPs) in response to ICA treatment. Western blot analysis was performed to validate the expression of DEPs. ICA administration promoted osteoblast viability, alkaline phosphatase activity, calcium deposition and inhibited osteoblast apoptosis. Secretome analysis of ICA‑treated cells was performed using two‑dimensional gel electrophoresis and matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry. A total of 56 DEPs were identified, including serpin family F member 1 (PEDF), protein disulfide isomerase family A, member 3 (PDIA3), nuclear protein, co‑activator of histone transcription (NPAT), c‑Myc and heat shock protein 70 (HSP70). These proteins were associated with signaling pathways, including Fas and p53. Bioinformatics and western blot analyses confirmed that the expression levels of the six DEPs were upregulated following ICA treatment. These genes may be directly or indirectly involved in ICA‑mediated osteogenic differentiation and osteogenesis. It was demonstrated that ICA treatment promoted osteogenesis by modulating the expression of PEDF, PDIA3, NPAT and HSP70 through signaling pathways, including Fas and p53.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Qian W, Su Y, Zhang Y, Yao N, Gu N, Zhang X and Yin H: Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17: 6515-6525, 2018.
APA
Qian, W., Su, Y., Zhang, Y., Yao, N., Gu, N., Zhang, X., & Yin, H. (2018). Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Molecular Medicine Reports, 17, 6515-6525. https://doi.org/10.3892/mmr.2018.8715
MLA
Qian, W., Su, Y., Zhang, Y., Yao, N., Gu, N., Zhang, X., Yin, H."Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis". Molecular Medicine Reports 17.5 (2018): 6515-6525.
Chicago
Qian, W., Su, Y., Zhang, Y., Yao, N., Gu, N., Zhang, X., Yin, H."Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis". Molecular Medicine Reports 17, no. 5 (2018): 6515-6525. https://doi.org/10.3892/mmr.2018.8715