Open Access

Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway

  • Authors:
    • Xin‑Guang Zhang
    • Zheng Xue
    • Yi‑Tao Zhao
    • Li Bai
    • Fei Liu
    • Li‑Qing Li
    • Jie Wu
    • Jing‑Dong Zhou
    • Jian‑Er Yu
  • View Affiliations

  • Published online on: March 14, 2018     https://doi.org/10.3892/mmr.2018.8747
  • Pages: 6977-6984
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to investigate the effects of liver soothing pingchuan formula decoction (LSPF) on experimental asthma in BALB/c mice and explore its potential molecular mechanisms. An animal model of asthma was established in BALB/c mice through sensitization and activation with intraperitoneal injection of 10% ovalbumin (OVA)/Al(OH)3 solution in addition to inhalation of a 5% OVA solution. LSPF (300 and 600 mg/kg/day) was initially administered orally prior to activation. Following this, bronchoalveolar lavage fluid (BALF) and lung tissues were collected for histopathalogical examination. Levels of inflammatory cells and cytokines were determined in the BALF, and levels of nerve growth factor (NGF) and tyrosine kinase A (TrkA) in the lung tissues were determined. The results of the present study indicated that increased inflammatory reactions were observed following OVA sensitization (P<0.05), and the expression levels of NGF (P<0.05) and TrkA (P<0.05) were significantly increased, compared with normal mice. Notably, compared with the asthma model group, immunohistochemical results revealed that LSPF treatment suppressed OVA induced inflammatory reactions (P<0.05) and NGF (P<0.05) and TrkA expression levels (P<0.05). In addition, the NGF (P<0.05) and TrkA (P<0.05) were revealed to be downregulated with LSPF treatment from the results of the ELISA and western blotting assay. Overall, the results of the present study demonstrated that LSPF exhibits therapeutic effects on experimental asthma in mice, via downregulation of the NGF-TrkA pathway.
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May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang XG, Xue Z, Zhao YT, Bai L, Liu F, Li LQ, Wu J, Zhou JD and Yu JE: Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway. Mol Med Rep 17: 6977-6984, 2018
APA
Zhang, X., Xue, Z., Zhao, Y., Bai, L., Liu, F., Li, L. ... Yu, J. (2018). Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway. Molecular Medicine Reports, 17, 6977-6984. https://doi.org/10.3892/mmr.2018.8747
MLA
Zhang, X., Xue, Z., Zhao, Y., Bai, L., Liu, F., Li, L., Wu, J., Zhou, J., Yu, J."Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway". Molecular Medicine Reports 17.5 (2018): 6977-6984.
Chicago
Zhang, X., Xue, Z., Zhao, Y., Bai, L., Liu, F., Li, L., Wu, J., Zhou, J., Yu, J."Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway". Molecular Medicine Reports 17, no. 5 (2018): 6977-6984. https://doi.org/10.3892/mmr.2018.8747