microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

  • Authors:
    • Xue‑Feng Zhang
    • Xue‑Qi Zhang
    • Zhe‑Xing Chang
    • Cui‑Cui Wu
    • Hang Guo
  • View Affiliations

  • Published online on: April 20, 2018     https://doi.org/10.3892/mmr.2018.8910
  • Pages: 8450-8456
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Abstract

MicroRNA (miRNA)‑145 has been demonstrated to serve a role in several types of tumors, however, the potential molecular mechanism of action of miRNA‑145 in bladder cancer metastasis remains to be elucidated. This study aimed to investigate the potential modulation of miRNA‑145 in bladder carcinoma and elucidate the underlying molecular mechanism. The expression of miRNA‑145 in bladder adenocarcinoma tissues and bladder cancer cells was measured by reverse transcription‑quantitative polymerase chain reaction. miRNA‑145 mimics and inhibitor were transfected into bladder cancer (BC) cells to determine the role of miRNA‑145 on cell motility and invasion measured by wound healing and transwell assays. Luciferase assay was performed to confirm whether N‑cadherin was the direct target of miRNA‑145. Subsequently, expression of N‑cadherin and matrix metalloproteinase‑9 (MMP9) in BC cells were detected by western blot analysis. miRNA‑145 was significantly downregulated cells and tissues from patients with BC, compared with healthy controls. miRNA‑145 markedly inhibited the ability of BC cells to migrate and invade. Furthermore, N‑cadherin was identified as a target of miRNA‑145 in BC cells. MMP9, acting downstream of N‑cadherin, was downregulated in BC cells by miRNA‑145. In the present study, miRNA‑145 suppressed the migration and invasion of BC cells by regulating N‑cadherin. The results of the present study indicated that miRNA‑145 may function as a tumor suppressor and may have a potential to be a diagnostic and predictive biomarker, and a therapeutic target for treatment of BC.
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June-2018
Volume 17 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang XF, Zhang XQ, Chang ZX, Wu CC and Guo H: microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin. Mol Med Rep 17: 8450-8456, 2018.
APA
Zhang, X., Zhang, X., Chang, Z., Wu, C., & Guo, H. (2018). microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin. Molecular Medicine Reports, 17, 8450-8456. https://doi.org/10.3892/mmr.2018.8910
MLA
Zhang, X., Zhang, X., Chang, Z., Wu, C., Guo, H."microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin". Molecular Medicine Reports 17.6 (2018): 8450-8456.
Chicago
Zhang, X., Zhang, X., Chang, Z., Wu, C., Guo, H."microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin". Molecular Medicine Reports 17, no. 6 (2018): 8450-8456. https://doi.org/10.3892/mmr.2018.8910