Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin

  • Authors:
    • Longbin Liu
    • Liping Meng
    • Peng Zhang
    • Hui Lin
    • Jufang Chi
    • Fang Peng
    • Hangyuan Guo
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  • Published online on: May 8, 2018     https://doi.org/10.3892/mmr.2018.8991
  • Pages: 429-434
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Abstract

Angiotensin II (Ang II) is reported to be involved in the development of various cardiovascular diseases by disrupting microvessel permeability, however, the underlying mechanism remains to be elucidated. The present study aimed to investigate the mechanism by which Ang II disrupts microvascular permeability. Rat endothelial cells were subjected to primary culture and identification. Cells in passages 4‑7 were then used for the following experiments. The cells were divided into control, Ang II, and Ang II + valsartan groups, and reverse transcription‑quantitative polymerase chain reaction and western blot analyses were perform to evaluate the expression of zonula occludens‑1 (ZO‑1) and vascular endothelial (VE)‑cadherin in the cells. The distribution of ZO‑1 protein was also detected using immunofluorescence assays. It was found that, compared with the control group, lower expression levels of ZO‑1 and VE‑cadherin were present in the Ang II group (P<0.01). ZO‑1 was also irregularly distributed at the periphery of the cells. In addition, the overexpression of VE‑cadherin reversed the effect of Ang II on the expression and distribution of ZO‑1 in endothelial cells. Together, these results suggested that Ang II inhibited the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin, thus destroying the tight junctions between endothelial cells, which may also be the mechanism by which Ang II is involved in the development of cardiovascular diseases.
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July-2018
Volume 18 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu L, Meng L, Zhang P, Lin H, Chi J, Peng F and Guo H: Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin. Mol Med Rep 18: 429-434, 2018
APA
Liu, L., Meng, L., Zhang, P., Lin, H., Chi, J., Peng, F., & Guo, H. (2018). Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin. Molecular Medicine Reports, 18, 429-434. https://doi.org/10.3892/mmr.2018.8991
MLA
Liu, L., Meng, L., Zhang, P., Lin, H., Chi, J., Peng, F., Guo, H."Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin". Molecular Medicine Reports 18.1 (2018): 429-434.
Chicago
Liu, L., Meng, L., Zhang, P., Lin, H., Chi, J., Peng, F., Guo, H."Angiotensin II inhibits the protein expression of ZO‑1 in vascular endothelial cells by downregulating VE‑cadherin". Molecular Medicine Reports 18, no. 1 (2018): 429-434. https://doi.org/10.3892/mmr.2018.8991