Identification of key gene networks associated with fracture healing using αSMA‑labeled progenitor cells

Wang,Hua; Wang,Yongxiang; He,Jinshan; Diao,Chunyu; Sun,Junying; Wang,Jingcheng

doi:10.3892/mmr.2018.9029

Identification of key gene networks associated with fracture healing using αSMA‑labeled progenitor cells

Authors:
- Hua Wang
- Yongxiang Wang
- Jinshan He
- Chunyu Diao
- Junying Sun
- Jingcheng Wang
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Affiliations: Department of Orthopedics, The First Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Orthopedics, Clinical Medical College of Yangzhou University, Subei People's Hospital of Jiangsu, Yangzhou, Jiangsu 225001, P.R. China
Published online on: May 17, 2018 https://doi.org/10.3892/mmr.2018.9029
Pages: 834-840
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the key gene network in fracture healing. The dataset GSE45156 was downloaded from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified using the linear models for microarray data package of Bioconductor. Subsequently, Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted for DEGs in day 2 and 6 fractured samples via the Database for Annotation, Visualization and Integrated Discovery. Furthermore, protein‑protein interactions (PPIs) of DEGs were analyzed and a PPI network was constructed. A total of 774 and 1,172 DEGs were identified in day 2 and 6 fractured samples, respectively, compared with unfractured controls. Of the DEGs in day 2 and 6 fractured samples, various upregulated DEGs, including protein kinase C α (Prkca) and B‑cell lymphoma antagonist/killer 1 were significantly enriched in GO terms associated with cell death, and certain downregulated DEGs, including fms‑related tyrosine kinase 1 (Flt1), nitric oxide synthase 3 (Nos3), bone morphogenetic protein 4 (Bmp4) and Notch1 were enriched in GO terms associated with angiogenesis. Furthermore, a series of downregulated DEGs were enriched in the Notch signaling pathway, including hes family bHLH transcription factor 1 and Notch1. Certain DEGs had a high degree and interacted with each other, including Flt1, Nos3, Bmp4 and Notch1, and Prkca and ras‑related C3 botulinum toxin substrate 3. The up and downregulated DEGs may exert critical functions by interactively regulating angiogenesis or apoptosis.

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