Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells

  • Authors:
    • Li‑Jing Ran
    • Yun Zeng
    • Shao‑Chun Wang
    • Di‑Si Zhang
    • Min Hong
    • Shao‑You Li
    • Jian Dong
    • Ming‑Xia Shi
  • View Affiliations

  • Published online on: May 23, 2018     https://doi.org/10.3892/mmr.2018.9053
  • Pages: 723-732
  • Copyright: © Ran et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of co‑culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells (AMSCs), to compare the expression of C‑X‑C motif chemokine receptor 4 (CXCR4) in co‑cultured AMSCs and to investigate the roles of the stromal cell‑derived factor‑1 (SDF‑1)/CXCR4 axis in the homing and migration of AMSCs. AMSCs were isolated from human amniotic membranes, purified and then differentiated into osteoblasts and adipocytes in vitro, which was verified by von Kossa Staining and Oil Red O staining. Cell viability was measured by Cell Counting kit‑8 and trypan blue assays at 24, 48 and 72 h, the expression of CXCR4 was analyzed by immunofluorescence‑based flow cytometry and reverse transcription‑quantitative polymerase chain reaction, and the migration ability of AMSCs in vitro was observed by a migration assay. The results demonstrated that cell viability (at 48 and 72 h) and survival (at 24, 48 and 72 h) in the co‑culture and serum groups were higher compared with the serum‑free group. Furthermore, CXCR4 mRNA and protein expression, and migration along the SDF‑1 gradient, in the co‑culture and serum‑free groups were higher compared with the serum group. Overall, the results indicated that AMSCs co‑cultured with AECs exhibited enhanced proliferation activity and survival rate. In conclusion, the present study demonstrated that co‑culture of AMSCs with AECs upregulated CXCR4 on the surface of AMSCs and enhanced the migration ability of AMSCs in vitro. This result may improve the directional migration and homing ability of AMSCs, as well as provide a theoretical basis for the application of AMSCs in clinical practice as a novel strategy to increase the success of hematopoietic stem cell transplantation.
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July-2018
Volume 18 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ran LJ, Zeng Y, Wang SC, Zhang DS, Hong M, Li SY, Dong J and Shi MX: Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Mol Med Rep 18: 723-732, 2018
APA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S. ... Shi, M. (2018). Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Molecular Medicine Reports, 18, 723-732. https://doi.org/10.3892/mmr.2018.9053
MLA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18.1 (2018): 723-732.
Chicago
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18, no. 1 (2018): 723-732. https://doi.org/10.3892/mmr.2018.9053