Protective effects of scoparone against ischemia‑reperfusion‑induced myocardial injury

Wan,Chunfu; Wei,Yueyue; Ma,Jianguo; Geng,Xiaoyong

doi:10.3892/mmr.2018.9123

Protective effects of scoparone against ischemia‑reperfusion‑induced myocardial injury

Authors:
- Chunfu Wan
- Yueyue Wei
- Jianguo Ma
- Xiaoyong Geng
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Affiliations: Department of Pain and Rehabilitation, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Urology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Cardiology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China
Published online on: June 4, 2018 https://doi.org/10.3892/mmr.2018.9123
Pages: 1752-1760

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Abstract

The present study aimed to investigate the protective effects and molecular mechanisms of scoparone on ischemia‑reperfusion (I/R) injury in primary cultured cardiac myocytes and rats. An in vivo rat model of I/R injury and an in vitro primary cultured cardiac myocyte model of oxygen‑glucose deprivation/reoxygenation were used to investigate the protective effects of scoparone. Cell viability, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD), creatine kinase (CK) and malondialdehyde (MDA) levels, and reactive oxygen species (ROS) production were subsequently measured. In addition, cell apoptosis was assessed by terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling staining, and myocardial infarct area (IA) was determined by triphenyl tetrazolium chloride staining. Furthermore, the protein expression levels of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), cytochrome c (Cyt C) and caspase‑3 were assessed by western blotting. The results demonstrated that treatment with scoparone markedly increased cell viability, SOD levels and Bcl‑2 protein expression, and decreased LDH release, MDA production, CK levels, ROS concentration, cell apoptotic rate, myocardial IA, and Bax, caspase‑3 and Cyt C protein expression. These findings indicated that scoparone may have a protective effect against I/R injury, thus suggesting that scoparone may be a considered a potential drug for the treatment of I/R injury via the inhibition of oxidative stress and cell apoptosis.

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1	Okuno I, Uchida K, Nakamura M and Sakurawi K: Studies on choleretic constituents in Artemisia capillaris THUNB. Chem Pharm Bull (Tokyo). 36:769–775. 1988. View Article : Google Scholar : PubMed/NCBI
2	Yoon M and Kim MY: The anti-angiogenic herbal composition Ob-X from Morus alba, Melissa officinalis and Artemisia capillaris regulates obesity in genetically obese ob/ob mice. Pham Biol. 49:614–619. 2011. View Article : Google Scholar
3	Ulicná O, Greksák M, Vancová O, Zlatos L, Galbavý S, Bozek P and Nakano M: Hepatoprotective effect of rooibos tea (Aspalathus linearis) on CCl4-induced liver damage in rats. Physiol Res. 52:461–466. 2003.PubMed/NCBI
4	Lee HI, Seo KO, Yun KW, Kim MJ and Lee MK: Comparative study of the hepatoprotective efficacy of Artemisia iwayomogi and Artemisia capillaris on ethanol-administered mice. J Food Sci. 76:T207–T211. 2011. View Article : Google Scholar : PubMed/NCBI
5	Jin X, Uchiyama M, Zhang Q, Watanabe T and Niimi M: Artemisiae capillaris herba induces prolonged survival of fully cardiac allografts and generates regulatory cells in mice. Transplant Proc. 44:1073–1075. 2012. View Article : Google Scholar : PubMed/NCBI
6	Kwon OS, Choi JS, Islam MN, Kim YS and Kim HP: Inhibition of 5-lipoxygenase and skin inflammation by the aerial parts of Artemisia capillaris and its constituents. Arch Pharm Res. 34:1561–1569. 2011. View Article : Google Scholar : PubMed/NCBI
7	Habib M and Waheed I: Evaluation of anti-nociceptive, anti-inflammatory and antipyretic activities of Artemisia scoparia hydromethanolic extract. J Ethnopharmacol. 145:18–24. 2013. View Article : Google Scholar : PubMed/NCBI
8	Jang SI, Kim YJ, Kim HJ, Lee JC, Kim HY, Kim YC, Yun YG, Yu HH and You YO: Scoparone inhibits PMA-induced IL-8 and MCP-1 production through suppression of NFkappaB activation in U937 cells. Life Sci. 78:2937–2943. 2006. View Article : Google Scholar : PubMed/NCBI
9	Jang SI, Kim YJ, Lee WY, Kwak KC, Baek SH, Kwak GB, Yun YG, Kwon TO, Chung HT and Chai KY: Scoparone from Artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by interferon-gamma Plus LPS. Arch Pharm Res. 28:203–208. 2005. View Article : Google Scholar : PubMed/NCBI
10	Kim JK, Kim JY, Kim HJ, Park KG, Harris RA, Cho WJ, Lee JT and Lee IK: Scoparone exerts anti-tumor activity against DU145 prostate cancer cells via inhibition of STAT3 activity. PLoS One. 8:e803912013. View Article : Google Scholar : PubMed/NCBI
11	Huang W, Zhang J and Moore DD: A traditional herbal medicine enhances bilirubin clearance by activating the nuclear receptor CAR. J Clin Invest. 113:137–143. 2004. View Article : Google Scholar : PubMed/NCBI
12	Huang HC, Weng YI, Lee CR, Jan TR, Chen YL and Lee YT: Protection by scoparone against the alterations of plasma lipoproteins, vascular morphology and vascular reactivity in hyperlipidaemic diabetic rabbit. Br J Pharmacol. 110:1508–1514. 1993. View Article : Google Scholar : PubMed/NCBI
13	Morisco C, Zebrowski D, Condorelli G, Tsichlis P, Vatner SF and Sadoshima J: The akt-glycogen synthase kinase 3beta pathway regulates transcription of atrial natriuretic factor induced by beta-adrenergic receptor stimulation in cardiac myocytes. J Biol Chem. 275:14466–14475. 2000. View Article : Google Scholar : PubMed/NCBI
14	Wu L, Qiao H, Li Y and Li L: Protective roles of puerarin and Danshensu on acute ischemic myocardial injury in rats. Phytomedicine. 14:652–658. 2007. View Article : Google Scholar : PubMed/NCBI
15	Yang J, Zhang XD, Yang J, Ding JW, Liu ZQ, Li SG and Yang R: The cardioprotective effect of fluvastatin on ischemic injury via down-regulation of toll-like receptor 4. Mol Biol Rep. 38:3037–3044. 2011. View Article : Google Scholar : PubMed/NCBI
16	Zhao ZY, Luan P, Huang SX, Xiao SH, Zhao J, Zhang B, Gu BB, Pi RB and Liu J: Edaravone protects HT22 neurons from H₂O₂-induced apoptosis by inhibiting the MAPK signaling pathway. CNS Neurosci Ther. 19:163–169. 2013. View Article : Google Scholar : PubMed/NCBI
17	Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI
18	Zhang L, Ma J and Liu H: Protective effect of ischemic postconditioning against ischemia reperfusion-induced myocardium oxidative injury in IR rats. Molecules. 17:3805–3817. 2012. View Article : Google Scholar : PubMed/NCBI
19	Goldhaber JI and Qayyum MS: Oxygen free radicals and excitation-contraction coupling. Antioxid Redox Signal. 2:55–64. 2000. View Article : Google Scholar : PubMed/NCBI
20	Paredies G, Pelrosillo G, Pistolese M, Di Venosa N, Federici A and Ruggiero FM: Decrease in mitoehondrial complex 1 activity in ischemic/reperfused rat heart: In-volvement of reactive oxygen species and eardiolipin. Cire Res. 94:53–59. 2004. View Article : Google Scholar
21	Zheng W, Huang LZ, Zhao L, Wang B, Xu HB, Wang GY, Wang ZL and Zhou H: Superoxide dismutase activity and malondialdehyde level in plasma and morphological evaluation of acute severe hemorrhagic shock in rats. Am J Emerg Med. 26:54–58. 2008. View Article : Google Scholar : PubMed/NCBI
22	Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD: The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis. Science. 275:1132–1136. 1997. View Article : Google Scholar : PubMed/NCBI
23	Tamm I, Schriever F and Dörken B: Apoptosis: Implications of basic research for clinical oncology. Lancet Oncol. 2:33–42. 2001. View Article : Google Scholar : PubMed/NCBI
24	Lakhani SA, Masud A, Kuida K, Porter GA Jr, Booth CJ, Mehal WZ, Inayat I and Flavell RA: Caspases 3 and 7: Key mediators of mitochondrial events of apoptosis. Science. 311:847–851. 2006. View Article : Google Scholar : PubMed/NCBI
25	Wu Q, Li H, Wu Y, Shen W, Zeng L, Cheng H and He L: Protective effects of muscone on ischemia-reperfusion injury in cardiac myocytes. J Ethnopharmacol. 138:34–39. 2011. View Article : Google Scholar : PubMed/NCBI
26	Song JQ and Liu YX: Effect of ramipril on ischemia -reperfusion induced apoptosis of cardiomyocyte and expression of apoptosis related genes. Chin Pharm Acol Bull. 22:625–628. 2006.