Expression of vitamin D receptor in bronchial asthma and its bioinformatics prediction

Shan,Lishen; Kang,Xinyuan; Liu,Fen; Cai,Xuxu; Han,Xiaohua; Shang,Yunxiao

doi:10.3892/mmr.2018.9157

Expression of vitamin D receptor in bronchial asthma and its bioinformatics prediction

Authors:
- Lishen Shan
- Xinyuan Kang
- Fen Liu
- Xuxu Cai
- Xiaohua Han
- Yunxiao Shang
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Affiliations: Department of Pediatric Pulmonology, Shengjing Hospital Affiliated to China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Orthopedics, Shengjing Hospital Affiliated to China Medical University, Shenyang, Liaoning 110122, P.R. China
Published online on: June 13, 2018 https://doi.org/10.3892/mmr.2018.9157
Pages: 2052-2060
Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Vitamin D receptors (VDRs) are associated with the occurrence and development of asthma. The aim of the present study was to analyze the secondary structure and B‑cell and T‑cell epitopes of VDR using online prediction software and aid in the future development of a highly efficient epitope‑based vaccine against asthma. Blood samples were collected from peripheral blood of asthmatic children. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) was performed to detect the expression of VDR in the peripheral blood. Mouse models of asthma were established. Hematoxylin and eosin staining was performed to observe the pathological alterations of the lungs of mice. Immunohistochemistry, western blot analysis and RT‑qPCR were performed to detect the expression of VDR in the lungs of asthmatic mice. Online prediction software immune epitope database and analysis resource, SYFPEITHI and linear epitope prediction based on propensity scale and support vector machines were used to predict the B‑cell and T‑cell epitopes and the RasMol and 3DLigandSite were used to analyze the tertiary structure of VDR. RT‑qPCR demonstrated that VDR expression in the peripheral blood of asthmatic children was decreased. Immunohistochemistry, western blotting and RT‑qPCR demonstrated that VDR expression also decreased in the lungs of mouse models of asthma. VDR B‑cell epitopes were identified at 37‑45, 88‑94, 123‑131, 231‑239, 286‑294 and 342‑350 positions of the amino acid sequence and VDR T‑cell epitopes were identified at 125‑130, 231‑239 and 265‑272 positions. A total of six B‑cell epitopes and three T‑cell epitopes for VDR were predicted by bioinformatics, which when validated, may in the future aid in immunological diagnosis and development of a targeted drug therapy for clinical asthma.

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