Open Access

Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells

  • Authors:
    • Yan Yu
    • Wanting Li
    • Liqin Ren
    • Chunyan Yang
    • Dongze Li
    • Xin Han
    • Yeying Sun
    • Changjun Lv
    • Fang Han
  • View Affiliations

  • Published online on: June 22, 2018     https://doi.org/10.3892/mmr.2018.9209
  • Pages: 2124-2132
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hypoxia is a type of cellular stress that may result in apoptosis and autophagy. The molecular mechanisms underlying the association between autophagy and apoptosis remain unclear, particularly in hypoxic conditions. Transmission electron microscope, AO‑PI staining, flow cytometry and western blot were used to examine the crosstalk between autophagy and apoptosis in hypoxic conditions. Rat alveolar type II epithelial RLE‑6TN cells were cultured in a long‑term hypoxic environment established by cobalt (II) chloride. It was demonstrated that autophagy and apoptosis occurred in RLE‑6TN cells under hypoxic conditions. Treatment of RLE‑6TN cells with the autophagy inhibitor 3‑methyladenine increased the generation of reactive oxygen species, mitochondrial damage and hypoxia‑induced apoptosis. The expression of caspases, particularly caspase‑9, increased and may have participated in these processes. The data indicated that the inhibition of autophagy enhanced apoptosis through the mitochondria‑mediated intrinsic pathway. These findings provide important insight into the molecular mechanism of autophagy and apoptosis crosstalk. This may provide new insights into pulmonary disease surveillance, diagnosis and treatment.
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August-2018
Volume 18 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yu Y, Li W, Ren L, Yang C, Li D, Han X, Sun Y, Lv C and Han F: Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells. Mol Med Rep 18: 2124-2132, 2018.
APA
Yu, Y., Li, W., Ren, L., Yang, C., Li, D., Han, X. ... Han, F. (2018). Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells. Molecular Medicine Reports, 18, 2124-2132. https://doi.org/10.3892/mmr.2018.9209
MLA
Yu, Y., Li, W., Ren, L., Yang, C., Li, D., Han, X., Sun, Y., Lv, C., Han, F."Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells". Molecular Medicine Reports 18.2 (2018): 2124-2132.
Chicago
Yu, Y., Li, W., Ren, L., Yang, C., Li, D., Han, X., Sun, Y., Lv, C., Han, F."Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells". Molecular Medicine Reports 18, no. 2 (2018): 2124-2132. https://doi.org/10.3892/mmr.2018.9209