Selective activation of inflammation factors by human parvovirus B19 and human bocavirus VP1 unique region on H9c2 cardiomyocyte

Hii,Hiong‑Ping; Chiu,Chun‑Ching; Lin,Di‑Wei; Shi,Ya‑Fang; Hsu,Tsai‑Ching; Tzang,Bor‑Show

doi:10.3892/mmr.2018.9369

Selective activation of inflammation factors by human parvovirus B19 and human bocavirus VP1 unique region on H9c2 cardiomyocyte

Authors:
- Hiong‑Ping Hii
- Chun‑Ching Chiu
- Di‑Wei Lin
- Ya‑Fang Shi
- Tsai‑Ching Hsu
- Bor‑Show Tzang
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Affiliations: Division of Cardiovascular, Department of Surgery, Chi Mei Medical Center, Tainan 710, Taiwan, R.O.C., Department of Neurology and Medical Intensive Care Unit, Chunghua Christian Hospital, Chunghua 505, Taiwan, R.O.C., Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University Hospital, Taichung 402, Taiwan, R.O.C.
Published online on: August 9, 2018 https://doi.org/10.3892/mmr.2018.9369
Pages: 4072-4078

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Abstract

Human parvovirus B19 (B19) and human bocavirus 1 (HBoV) are the only known pathogenic parvoviruses, and are responsible for a variety of diseases in human beings. Mounting evidence indicates a strong association between B19 infection and cardiac disorders including myocarditis, dilated cardiomyopathy and heart failure. However, very limited information about the role of HBoV in cardiac disorders is known. To elucidate the effects of B19 and HBoV on cardiac disorders, we expressed EGFP‑conjugate constructs of B19‑VP1 unique region (VP1u) and HBoV‑VP1u, along with the mutants EGFP‑B19‑VP1uD175A and EGFP‑HBoV‑VP1uV12A, in H9c2 cells by stable transfection. The protein expression levels of EGFP, EGFP‑B19‑VP1u, EGFP‑B19‑VP1uD175A, EGFP‑HBoV‑VP1u and EGFP‑HBoV‑VP1uV12A in H9c2 cells were observed under a fluorescence microscope and confirmed by western blotting. Secreted phospholipase A2 (sPLA2) activity was detected in B19‑VP1u and HBoV‑VP1u but not B19‑VP1uD175A and HBoV‑VP1uV12A recombinant proteins. Significantly higher expression levels of MCP2 and IP‑10 mRNA were detected in H9c2 cells that were transfected with pEGFP‑B19‑VP1u, compared with in those cells transfected with pEGFP‑HBoV‑VP1u, pEGFP‑B19‑VP1uD175A or pEGFP‑HBoV‑VP1uV12A. Significantly higher protein levels of IL‑1β and IL‑6 were detected in H9c2 cells transfected with pEGFP‑B19‑VP1u or pEGFP‑HBoV‑VP1u, compared with in those cells transfected with pEGFP‑B19‑VP1uD175A or pEGFP‑HBoV‑VP1uV12A. Notably, significantly higher expression of both TNF‑α and NF‑κB was observed only in H9c2 cells transfected with pEGFP‑B19‑VP1u, but not in those cells transfected with pEGFP‑HBoV‑VP1u, pEGFP‑B19‑VP1uD175A or pEGFP‑HBoV‑VP1uV12A. These findings, to our knowledge for the first time, reveal the difference between B19‑VP1u and HBoV‑VP1u in H9c2 cells and provide insight into the roles of B19‑VP1u and HBoV‑VP1u in the pathogenesis of cardiac inflammation.

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1	Berns KI and Parrish CR: ParvoviridaeFields Virology. Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR and Roizman B: 6th. Lippincott Williams & Wilkins; Philadelphia, PA: pp. 1768–1791. 2013
2	Qiu J, Söderlund-Venermo M and Young NS: Human parvoviruses. Clin Microbiol Rev. 30:43–113. 2017. View Article : Google Scholar : PubMed/NCBI
3	Cossart YE, Field AM, Cant B and Widdows D: Parvovirus-like particles in human sera. Lancet. 1:72–73. 1975. View Article : Google Scholar : PubMed/NCBI
4	Brown KE and Young NS: Parvovirus B19 in human disease. Annu Rev Med. 48:59–67. 1997. View Article : Google Scholar : PubMed/NCBI
5	Heegaard ED and Brown KE: Human parvovirus B19. Clin Microbiol Rev. 15:485–505. 2002. View Article : Google Scholar : PubMed/NCBI
6	Young NS and Brown KE: Parvovirus B19. N Engl J Med. 350:586–597. 2004. View Article : Google Scholar : PubMed/NCBI
7	Page C, François C, Goëb V and Duverlie G: Human parvovirus B19 and autoimmune diseases. Review of the literature and pathophysiological hypotheses. J Clin Virol. 72:69–74. 2015. View Article : Google Scholar : PubMed/NCBI
8	Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A and Andersson B: Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA. 102:pp. 12891–12896. 2005; View Article : Google Scholar : PubMed/NCBI
9	Jartti T, Hedman K, Jartti L, Ruuskanen O, Allander T and Söderlund-Venermo M: Human bocavirus-the first 5 years. Rev Med Virol. 22:46–64. 2012. View Article : Google Scholar : PubMed/NCBI
10	Naides SJ and Weiner CP: Antenatal diagnosis and palliative treatment of non-immune hydrops fetalis secondary to fetal parvovirus B19 infection. Prenat Diagn. 9:105–114. 1989. View Article : Google Scholar : PubMed/NCBI
11	Feldman AM and McNamara D: Myocarditis. N Engl J Med. 343:1388–1398. 2000. View Article : Google Scholar : PubMed/NCBI
12	Murry CE, Jerome KR and Reichenbach DD: Fatal parvovirus myocarditis in a 5-year-old girl. Hum Pathol. 32:342–345. 2001. View Article : Google Scholar : PubMed/NCBI
13	Lamparter S, Schoppet M, Pankuweit S and Maisch B: Acute parvovirus B19 infection associated with myocarditis in an immunocompetent adult. Hum Pathol. 34:725–728. 2003. View Article : Google Scholar : PubMed/NCBI
14	Kühl U, Pauschinger M, Bock T, Klingel K, Schwimmbeck CP, Seeberg B, Krautwurm L, Poller W, Schultheiss HP and Kandolf R: Parvovirus B19 infection mimicking acute myocardial infarction. Circulation. 108:945–950. 2003. View Article : Google Scholar : PubMed/NCBI
15	Pankuweit S, Moll R, Baandrup U, Portig I, Hufnagel G and Maisch B: Prevalence of the parvovirus B19 genome in endomyocardial biopsy specimens. Hum Pathol. 34:497–503. 2003. View Article : Google Scholar : PubMed/NCBI
16	Pankuweit S, Lamparter S, Schoppet M and Maisch B: Parvovirus B19 genome in endomyocardial biopsy specimen. Circulation. 109:e1792004. View Article : Google Scholar : PubMed/NCBI
17	Dorsch S, Liebisch G, Kaufmann B, von Landenberg P, Hoffmann JH, Drobnik W and Modrow S: The VP1 unique region of parvovirus B19 and its constituent phospholipase A2-like activity. J Virol. 76:2014–2018. 2002. View Article : Google Scholar : PubMed/NCBI
18	Filippone C, Zhi N, Wong S, Lu J, Kajigaya S, Gallinella G, Kakkola L, Söderlund-Venermo M, Young NS and Brown KE: VP1u phospholipase activity is critical for infectivity of full-length parvovirus B19 genomic clones. Virology. 374:444–452. 2008. View Article : Google Scholar : PubMed/NCBI
19	Leisi R, Ruprecht N, Kempf C and Ros C: Parvovirus B19 uptake is a highly selective process controlled by VP1u, a novel determinant of viral tropism. J Virol. 87:13161–13167. 2013. View Article : Google Scholar : PubMed/NCBI
20	Hsu TC, Tsai CC, Chiu CC, Hsu JD and Tzang BS: Exacerbating effects of human parvovirus B19 NS1 on liver fibrosis in NZB/W F1 mice. PLoS One. 8:e683932013. View Article : Google Scholar : PubMed/NCBI
21	Tsai CC, Chiu CC, Hsu JD, Hsu HS, Tzang BS and Hsu TC: Human parvovirus B19 NS1 protein aggravates liver injury in NZB/W F1 mice. PLoS One. 8:e597242013. View Article : Google Scholar : PubMed/NCBI
22	Nie X, Zhang G, Xu D, Sun X, Li Z, Li X, Zhang X, He F and Li Y: The VP1-unique region of parvovirus B19 induces myocardial injury in mice. Scand J Infect Dis. 42:121–128. 2010. View Article : Google Scholar : PubMed/NCBI
23	Tzang BS, Lin TM, Tsai CC, Hsu JD, Yang LC and Hsu TC: Increased cardiac injury in NZB/W F1 mice received antibody against human parvovirus B19 VP1 unique region protein. Mol Immunol. 48:1518–1524. 2011. View Article : Google Scholar : PubMed/NCBI
24	Bogomolovas J, Šimoliūnas E, Rinkūnaitė I, Smalinskaitė L, Podkopajev A, Bironaitė D, Weis CA, Marx A, Bukelskienė V, Gretz N, et al: A novel murine model of parvovirus associated dilated cardiomyopathy induced by immunization with VP1-unique region of parvovirus B19. Biomed Res Int. 2016:16271842016. View Article : Google Scholar : PubMed/NCBI
25	Qu XW, Liu WP, Qi ZY, Duan ZJ, Zheng LS, Kuang ZZ, Zhang WJ and Hou YD: Phospholipase A2-like activity of human bocavirus VP1 unique region. Biochem Biophys Res Commun. 365:158–163. 2008. View Article : Google Scholar : PubMed/NCBI
26	Tzang BS, Tsay GJ, Lee YJ, Li C, Sun YS and Hsu TC: The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production. Clin Chim Acta. 378:59–65. 2007. View Article : Google Scholar : PubMed/NCBI
27	Chiu CC, Shi YF, Yang JJ, Hsiao YC, Tzang BS and Hsu TC: Effects of human Parvovirus B19 and Bocavirus VP1 unique region on tight junction of human airway epithelial A549 cells. PLoS One. 9:e1079702014. View Article : Google Scholar : PubMed/NCBI
28	Zádori Z, Szelei J, Lacoste MC, Li Y, Gariépy S, Raymond P, Allaire M, Nabi IR and Tijssen P: A viral phospholipase A2 is required for parvovirus infectivity. Dev Cell. 1:291–302. 2001. View Article : Google Scholar : PubMed/NCBI
29	Bonnerot C, Rocancourt D, Briand P, Grimber G and Nicolas JF: A beta-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies. Proc Natl Acad Sci USA. 84:pp. 6795–6799. 1987; View Article : Google Scholar : PubMed/NCBI
30	Valaperti A: Drugs targeting the canonical NF-κB pathway to treat viral and autoimmune myocarditis. Curr Pharm Des. 22:440–449. 2016. View Article : Google Scholar : PubMed/NCBI
31	Gullestad L, Ueland T, Vinge LE, Finsen A, Yndestad A and Aukrust P: Inflammatory cytokines in heart failure: Mediators and markers. Cardiology. 122:23–35. 2012. View Article : Google Scholar : PubMed/NCBI
32	Feldman AM, Combes A, Wagner D, Kadakomi T, Kubota T, Li YY and McTiernan C: The role of tumor necrosis factor in the pathophysiology of heart failure. J Am Coll Cardiol. 35:537–544. 2000. View Article : Google Scholar : PubMed/NCBI
33	Frangogiannis NG: Chemokines in the ischemic myocardium: From inflammation to fibrosis. Inflamm Res. 53:585–595. 2004. View Article : Google Scholar : PubMed/NCBI
34	Braunersreuther V, Mach F and Steffens S: The specific role of chemokines in atherosclerosis. Thromb Haemost. 97:714–721. 2007. View Article : Google Scholar : PubMed/NCBI
35	Ardigo D, Assimes TL, Fortmann SP, Go AS, Hlatky M, Hytopoulos E, Iribarren C, Tsao PS, Tabibiazar R and Quertermous T; ADVANCE Investigators, : Circulating chemokines accurately identify individuals with clinically significant atherosclerotic heart disease. Physiol Genomics. 31:402–409. 2007. View Article : Google Scholar : PubMed/NCBI
36	Ros C, Gerber M and Kempf C: Conformational changes in the VP1-unique region of native human parvovirus B19 lead to exposure of internal sequences that play a role in virus neutralization and infectivity. J Virol. 80:12017–12024. 2006. View Article : Google Scholar : PubMed/NCBI