MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion

Xiao,Qi; Chen,Tianming; Wu,Yao; Wu,Wenxiu; Xu,Yandi; Gong,Zhunan; Chen,Shilin

doi:10.3892/mmr.2018.9372

MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion

Authors:
- Qi Xiao
- Tianming Chen
- Yao Wu
- Wenxiu Wu
- Yandi Xu
- Zhunan Gong
- Shilin Chen
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Affiliations: School of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210046, P.R. China, Department of Surgery, Nanjing Medical University Third Affiliated Hospital, Nanjing, Jiangsu 211166, P.R. China, Department of Thoracic Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China
Published online on: August 9, 2018 https://doi.org/10.3892/mmr.2018.9372
Pages: 3631-3640
Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal cancer ranks fourth in cancer‑associated mortality in China and the incidence of esophageal adenocarcinoma has risen dramatically over the past two decades. MicroRNA (miRNA/miR) serves a pivotal role in human cancer cell growth, invasion and migration. MiR‑675‑3p is highly expressed in esophageal squamous cell cancer (ESCC) tissues, and may have an influence on ESCC cell migration and invasion. ESCC tumor tissue samples from 35 patients were proﬁled. MiR‑675‑3p expression was confirmed by reverse transcription‑quantitative polymerase chain reaction. Manipulation of miR‑675‑3p via knockdown was carried out with subsequent evaluation of effects on cell proliferation, invasion, migration, and use of western blotting and ELISA assays. MiR‑675‑3p was overexpressed in ESCC tissues compared with normal tissues, and had higher expression levels in ESCC cells compared with the healthy esophageal epithelial cell line. The results revealed a predominant upregulation of cell migration and invasion ability. MiR‑675‑3p inhibitor inhibited ESCC cell proliferation, migration and invasion ability. It was also demonstrated that downregulation of miR‑675‑3p decreased the levels of matrix metalloproteinase (MMP) 2 and 9 and increased the level of E‑cadherin. In addition, the effects of miR‑675‑3p inhibitor on ESCC cell lines were eliminated by con‑transfection with miR‑675‑3p inhibitor and miR‑675‑3p mimic. In conclusion, the results indicated that miR‑675‑3p may be involved in the progression of ESCC through regulating ESCC cell migration and invasion capacity via modulating epithelial mesenchymal transition markers (MMP2, MMP 9 and E‑cadherin).

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