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An effective range of polydeoxyribonucleotides is critical for wound healing quality

  • Authors:
    • Kyu‑Hee Hwang
    • Ji‑Hee Kim
    • Eun Young Park
    • Seung‑Kuy Cha

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  • Published online on: October 8, 2018     https://doi.org/10.3892/mmr.2018.9539
  • Pages: 5166-5172
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50‑1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN‑mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN‑injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50‑1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c‑Jun N‑terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.
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December-2018
Volume 18 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Hwang KH, Kim JH, Park EY and Cha SK: An effective range of polydeoxyribonucleotides is critical for wound healing quality. Mol Med Rep 18: 5166-5172, 2018.
APA
Hwang, K., Kim, J., Park, E.Y., & Cha, S. (2018). An effective range of polydeoxyribonucleotides is critical for wound healing quality. Molecular Medicine Reports, 18, 5166-5172. https://doi.org/10.3892/mmr.2018.9539
MLA
Hwang, K., Kim, J., Park, E. Y., Cha, S."An effective range of polydeoxyribonucleotides is critical for wound healing quality". Molecular Medicine Reports 18.6 (2018): 5166-5172.
Chicago
Hwang, K., Kim, J., Park, E. Y., Cha, S."An effective range of polydeoxyribonucleotides is critical for wound healing quality". Molecular Medicine Reports 18, no. 6 (2018): 5166-5172. https://doi.org/10.3892/mmr.2018.9539