Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats

Xing,Pengcheng; Ma,Ke; Wu,Jun; Long,Wei; Wang,Donglian

doi:10.3892/mmr.2018.9579

Protective effect of polysaccharide peptide on cerebral ischemia‑reperfusion injury in rats

Authors:
- Pengcheng Xing
- Ke Ma
- Jun Wu
- Wei Long
- Donglian Wang
View Affiliations

Affiliations: Department of Emergency, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China, ICU, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China, Department of Geriatric Medicine, Shanghai Sixth People's Hospital East Area Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 201306, P.R. China
Published online on: October 23, 2018 https://doi.org/10.3892/mmr.2018.9579
Pages: 5371-5378
Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In the present study, the protective effects and regulatory mechanism of polysaccharide peptide (PSP) were investigated in rats with cerebral ischemia‑reperfusion (IR) injury. Neuroblastoma N2a cells were divided into five groups: Negative control; IR injury; PSP low dose treatment; PSP middle dose treatment; and PSP high dose treatment. In vitro, the cell viability was detected by an MTT assay. ELISA was performed to determine the activity of lactate dehydrogenase (LDH) and caspase‑3. A cerebral IR injury model in vivo was established, and hematoxylin and eosin (H&E) staining, western blotting, neurological deficit score and cerebral infarction were assessed. The cell viability was markedly improved following treatment with PSP and the activity of LDH and caspase‑3 was decreased following PSP administration (P<0.05). The in vivo studies determined that the neurological deficit score and cerebral infarction volume were reduced with the concentration of PSP increasing between 150 and 250 mg/kg. The H&E staining indicated that PSP was able to protect the nerve cells against the cerebral IR injury. In addition, PSP upregulated the decreased silent information regulator protein 1, peroxisome proliferator‑activated receptor γ coactivator‑1α and apoptosis regulator B‑cell lymphoma 2 expression induced by cerebral IR injury. The protein expression level of caspase‑3 and apoptosis regulator apoptosis regulator Bcl‑2‑like protein 4 was downregulated following PSP administration. These results suggested that PSP may improve nerve cell viability, enhance the neuroprotective role in cerebral IR injury and provide a novel approach for the treatment of cerebral IR injury.

View Figures

View References

1	Yamashita T and Abe K: Recent progress in therapeutic strategies for ischemic stroke. Cell Transplant. 25:893–898. 2016. View Article : Google Scholar : PubMed/NCBI
2	Dong Q, Lin X, Shen L and Feng Y: The protective effect of herbal polysaccharides on ischemia-reperfusion injury. Int J Biol Macromol. 92:431–440. 2016. View Article : Google Scholar : PubMed/NCBI
3	Rao PR, Kumar VK, Viswanath RK and Subbaraju GV: Cardioprotective activity of alcoholic extract of Tinospora cordifolia in ischemia reperfusion induced myocardial infarction in rats. Biol Pharm Bull. 28:2319–2322. 2005. View Article : Google Scholar : PubMed/NCBI
4	Kalogeris T, Baines CP, Krenz M and Korthuis RJ: Cell biology of ischemia/reperfusion injury. Int Rev Cell Mol Biol. 298:229–317. 2012. View Article : Google Scholar : PubMed/NCBI
5	Zerna C, Assis Z, d'Esterre CD, Menon BK and Goyal M: Imaging, intervention, and workflow in acute ischemic stroke: The calgaryapproach. AJNR Am J Neuroradiol. 37:978–984. 2016. View Article : Google Scholar : PubMed/NCBI
6	Sugawara T and Chan PH: Reactive oxygen radicals and pathogenesis of neuronal death after cerebral ischemia. Antioxid Redox Signal. 5:597–607. 2003. View Article : Google Scholar : PubMed/NCBI
7	Adibhatla Muralikrishna R and Hatcher JF: Phospholipase A2, reactive oxygen species, and lipid peroxidation in cerebral ischemia. Free Radic Biol Med. 40:376–387. 2006. View Article : Google Scholar : PubMed/NCBI
8	Xu M, Wang HF, Zhang YY and Zhuang HW: Protection of rats spinal cord ischemia-reperfusion injury by inhibition of MiR497 on inflammation and apoptosis: Possible role in pediatrics. Biomed Pharmacother. 81:337–344. 2016. View Article : Google Scholar : PubMed/NCBI
9	Zhu T, Yao Q, Hu X, Chen C, Yao H and Chao J: The role of MCPIP1 in ischemia/reperfusion injury-induced HUVEC migrationand apoptosis. Cell Physiol Biochem. 37:577–591. 2015. View Article : Google Scholar : PubMed/NCBI
10	Arda-Pirincci P and Bolkent S: The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats. Acta Histochem. 116:167–175. 2014. View Article : Google Scholar : PubMed/NCBI
11	Shokeir AA, Barakat N, Hussein AM, Awadalla A, Harraz AM, Khater S, Hemmaid K and Kamal AI: Activation of Nrf2 by ischemic preconditioning and sulforaphane in renalischemia/reperfusion injury: A comparative experimental study. Physiol Res. 64:313–323. 2015.PubMed/NCBI
12	Ju J, Wu J and Hou R: Role of the p38 mitogen-activated protein kinase signaling pathway inestrogen-mediated protection following flap ischemia-reperfusion injury. Cell Biochem Funct. 34:522–530. 2016. View Article : Google Scholar : PubMed/NCBI
13	Kovalska M, Kovalska L, Pavlikova M, Janickova M, Mikuskova K, Adamkov M, Kaplan P, Tatarkova Z and Lehotsky J: Intracellular signaling MAPK pathway after cerebral ischemia-reperfusion injury. Neurochem Res. 37:1568–1577. 2012. View Article : Google Scholar : PubMed/NCBI
14	Imahashi K, Schneider MD, Steenbergen C and Murphy E: Transgenic expression of Bcl-2 modulates energy metabolism, preventscytosolic acidification during ischemia, and reduces ischemia/reperfusion injury. Circ Res. 95:734–741. 2004. View Article : Google Scholar : PubMed/NCBI
15	Wei DF, Chen T, Yan M, Zhao W, Li F, Cheng W and Yuan L: Synthesis, characterization, antioxditant activity and neuroprotective effects of selenium polysaccharide from Radix hedysari. Carbohydr Polym. 125:161–168. 2015. View Article : Google Scholar : PubMed/NCBI
16	Sanagi MM, Loh SH, Ibrahim Wan WN, Pourmand N, Salisu A, Ibrahim Wan WA and Ali I: Agarose- and alginate based biopolymers for sample preparation: Excellentgreen extraction tools for this century. J Sep Sci. 39:1152–1159. 2016. View Article : Google Scholar : PubMed/NCBI
17	Gong J, Sun F, Li Y, Zhou X, Duan Z, Duan F, Zhao L, Chen H, Qi S and Shen J: Momordica charantia polysaccharides could protect against cerebral ischemia/reperfusion injury through inhibiting oxidative stress mediated c-Jun N-terminal kinase 3 signaling pathway. Neuropharmacology. 91:123–134. 2015. View Article : Google Scholar : PubMed/NCBI
18	Zhou ZY, Tang YP, Xiang J, Wua P, Jin HM, Wang Z, Mori M and Cai DF: Neuroprotective effects of water-soluble ganoderma lucidum polysaccharideson cerebral ischemic injury in rats. J Ethno pharmacol. 131:154–164. 2010. View Article : Google Scholar
19	Lu ZQ, Deng YJ and Lu JX: Effect of aloe polysaccharide on caspase-3 expression following cerebral ischemia and reperfusion injury in rats. Mol Med Rep. 6:371–374. 2012. View Article : Google Scholar : PubMed/NCBI
20	Zhang S, He B, Ge J, Zhai C, Liu X and Liu P: Characterization of chemical composition of agaricus brasiliensis polysaccharides and its effect on myocardial SOD activity, MDA and caspase-3 level in ischemia-reperfusion rats. Int J Biol Macromol. 46:363–366. 2010. View Article : Google Scholar : PubMed/NCBI
21	Reis C, Wang Y, Akyol O, Ho WM, Ii RA, Stier G, Martin R and Zhang JH: What's new in traumatic brain injury: Update on tracking, monitoring and treatment. Int J Mol Sci. 16:11903–11965. 2015. View Article : Google Scholar : PubMed/NCBI
22	Shi P, Zhang L, Wang J, Lu D, Li Y, Ren J, Shen M, Zhang L and Huang J: Porcine FcεRI mediates porcine reproductive and respiratory syndrome virus multiplication and regulates the inflammatory reaction. Virol Sin. 33:249–260. 2018. View Article : Google Scholar
23	Bösel J, Ruscher K, Ploner CJ and Valdueza JM: Delayed neurological deterioration in a stroke patient with postoperative acute anemia. Eur Neurol. 53:36–38. 2005. View Article : Google Scholar : PubMed/NCBI
24	Haigis MC and Sinclair DA: Mammalian sirtuins: Biological insights and disease relevance. Annu Rev Pathol. 5:253–295. 2010. View Article : Google Scholar : PubMed/NCBI
25	Zille M, Farr TD, Przesdzing I, Müller J, Sommer C, Dirnagl U and Wunder A: Visualizing cell death in experimental focal cerebral ischemia: Promises, problems, and perspectives. J Cereb Blood Flow Metab. 32:213–231. 2012. View Article : Google Scholar : PubMed/NCBI
26	Hyman BT and Yuan J: Apoptotic and non apoptotic roles of caspases in neuronal physiology and pathophysiology. Nat Rev Neurosci. 13:395–406. 2012. View Article : Google Scholar : PubMed/NCBI
27	Elmore S: Apoptosis: A review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI
28	Walsh JG, Cullen SP, Sheridan C, Lüthi AU, Gerner C and Martin SJ: Executioner caspase-3 and caspase-7 are functionally distinct proteases. Proc Natl Acad Sci USA. 105:12815–12819. 2008. View Article : Google Scholar : PubMed/NCBI
29	Manabat C, Han BH, Wendland M, Derugin N, Fox CK, Choi J, Holtzman DM, Ferriero DM and Vexler ZS: Reperfusion differentially induces caspase-3 activation in ischemic core and penumbra after stroke in immature brain. Stroke. 34:207–213. 2003. View Article : Google Scholar : PubMed/NCBI
30	Ferrer I and Planas AM: Signaling of cell death and cell survival following focal cerebral ischemi: Lifeand death struggle in the penumbra. J Neuropathol Exp Neurol. 62:329–339. 2003. View Article : Google Scholar : PubMed/NCBI
31	Potente M and Dimmeler S: Emerging roles of SIRT1 in vascular endothelial homeostasis. Cell Cycle. 7:2117–2122. 2008. View Article : Google Scholar : PubMed/NCBI
32	Maiese K, Chong ZZ, Shang YC and Wang S: Translating cell survival and cell longevity into treatment strategies with SIRT1. Rom J Morphol Embryol. 52:1173–1185. 2011.PubMed/NCBI
33	Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM and Puigserver P: Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 434:113–118. 2005. View Article : Google Scholar : PubMed/NCBI
34	Xia N, Förstermann U and Li HG: Resveratrol and endothelial nitric oxide. Molecules. 19:16102–16121. 2014. View Article : Google Scholar : PubMed/NCBI
35	Feng J, Yang Y, Zhou Y, Wang B, Xiong H, Fan C, Jiang S, Liu J, Ma Z, Hu W, et al: Bakuchiol attenuates myocardial ischemia reperfusion injury by maintaining mitochondrial function: The role of silent information regulator 1. Apoptosis. 21:532–545. 2016. View Article : Google Scholar : PubMed/NCBI
36	Tsai KL, Huang PH, Kao CL, Leu HB, Cheng YH, Liao YW, Yang YP, Chien Y, Wang CY, Hsiao CY, et al: Aspirin attenuates vinorelbine-induced endothelial inflammation via modulating SIRT1/AMPK axis. Biochem Pharmacol. 88:189–200. 2014. View Article : Google Scholar : PubMed/NCBI
37	Tamaki N, Cristina Orihuela-Campos R, Inagaki Y, Fukui M, Nagata T and Ito HO: Resveratrol improves oxidative stress and prevents the progression of periodontitis via the activation of the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in a rat periodontitis model. Free Radic Biol Med. 75:222–229. 2014. View Article : Google Scholar : PubMed/NCBI
38	Liu S, Xu J, Fang C, Shi C, Zhang X, Yu B and Yin Y: Over-expression of heat shock protein 70 protects mice against lung ischemia/reperfusion injury through SIRT1/AMPK/eNOS pathway. Am J Transl Res. 8:4394–4404. 2016.PubMed/NCBI