Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury

Li,Shuping; He,Jiaxuan

doi:10.3892/mmr.2018.9582

Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury

Authors:
- Shuping Li
- Jiaxuan He
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Affiliations: Anesthesiology Department, Xinjiang Uygur Autonomous Region Hospital of TCM, Urumchi, Xinjiang 830000, P.R. China, Anesthesiology Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
Published online on: October 24, 2018 https://doi.org/10.3892/mmr.2018.9582
Pages: 5353-5360
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pilose antler polypeptide (PAP) is an active substance isolated from the traditional Chinese medicine pilose antler, which possesses multiple biological activities. In the present study, the role and mechanism of PAP in sevoflurane (SEV)‑induced neurocyte injury was explored. Cell viability was determined by Cell Counting kit‑8 assay. Cell proliferation and apoptosis were analyzed by flow cytometry. Western blotting and reverse transcription‑quantitative polymerase chain reaction analysis were used to evaluate the protein and mRNA expression levels, respectively. The results revealed that PAP enhanced the cell viability of SEV‑treated nerve cells. In addition, through modulation of apoptosis‑associated protein expression, PAP suppressed SEV‑induced nerve cell apoptosis. Furthermore, PAP activated the p38 mitogen‑activated protein kinase (p38)/c‑Jun N‑terminal kinase (JNK) pathway in the neurocyte injury model, whereas inhibition of the p38/JNK pathway reversed the beneficial effects produced by PAP. In conclusion, PAP protected against SEV‑mediated neurocyte injury via upregulation of the p38/JNK pathway. The present findings suggested that PAP may be an effective agent for neurocyte injury.

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1	Cornelissen L, Kim SE, Purdon PL, Brown EN and Berde CB: Age-dependent electroencephalogram (EEG) patterns during sevoflurane general anesthesia in infants. Elife. 4:e065132015. View Article : Google Scholar : PubMed/NCBI
2	Dehaene-Lambertz G and Spelke ES: The infancy of the human brain. Neuron. 88:93–109. 2015. View Article : Google Scholar : PubMed/NCBI
3	Lee JH, Zhang J, Wei L and Yu SP: Neurodevelopmental implications of the general anesthesia in neonate and infants. Exp Neurol. 272:50–60. 2015. View Article : Google Scholar : PubMed/NCBI
4	Lerman J, Sikich N, Kleinman S and Yentis S: The pharmacology of sevoflurane in infants and children. Anesthesiology. 80:814–824. 1994. View Article : Google Scholar : PubMed/NCBI
5	Lee JR, Lin EP, Hofacer RD, Upton B, Lee SY, Ewing L, Joseph B and Loepke AW: Alternative technique or mitigating strategy for sevoflurane-induced neurodegeneration: A randomized controlled dose-escalation study of dexmedetomidine in neonatal rats. Br J Anaesth. 119:492–505. 2017. View Article : Google Scholar : PubMed/NCBI
6	Bignami E, Biondi-Zoccai G, Landoni G, Fochi O, Testa V, Sheiban I, Giunta F and Zangrillo A: Volatile anesthetics reduce mortality in cardiac surgery. J Cardiothorac Vasc Anesth. 23:594–599. 2009. View Article : Google Scholar : PubMed/NCBI
7	Lunardi N, Ori C, Erisir A and Jevtovic-Todorovic V: General anesthesia causes long-lasting disturbances in the ultrastructural properties of developing synapses in young rats. Neurotox Res. 17:179–188. 2010. View Article : Google Scholar : PubMed/NCBI
8	Tu S, Wang X, Yang F, Chen B, Wu S, He W, Yuan X, Zhang H, Chen P and Wei G: Propofol induces neuronal apoptosis in infant rat brain under hypoxic conditions. Brain Res Bull. 86:29–35. 2011. View Article : Google Scholar : PubMed/NCBI
9	Briner A, De Roo M, Dayer A, Muller D, Habre W and Vutskits L: Volatile anesthetics rapidly increase dendritic spine density in the rat medial prefrontal cortex during synaptogenesis. Anesthesiology. 112:546–556. 2010. View Article : Google Scholar : PubMed/NCBI
10	Briner A, Nikonenko I, De Roo M, Dayer A, Muller D and Vutskits L: Developmental stage-dependent persistent impact of propofol anesthesia on dendritic spines in the rat medial prefrontal cortex. Anesthesiology. 115:282–293. 2011. View Article : Google Scholar : PubMed/NCBI
11	Wang Y, Cheng Y, Liu G, Tian X, Tu X and Wang J: Chronic exposure of gestation rat to sevoflurane impairs offspring brain development. Neurol Sci. 33:535–544. 2012. View Article : Google Scholar : PubMed/NCBI
12	Mintz CD, Barrett KM, Smith SC, Benson DL and Harrison NL: Anesthetics interfere with axon guidance in developing mouse neocortical neurons in vitro via a γ-aminobutyric acid type A receptor mechanism. Anesthesiology. 118:825–833. 2013. View Article : Google Scholar : PubMed/NCBI
13	Mintz CD, Smith SC, Barrett KM and Benson DL: Anesthetics interfere with the polarization of developing cortical neurons. J Neurosurg Anesthesiol. 24:368–375. 2012. View Article : Google Scholar : PubMed/NCBI
14	Arbabi S and Maier RV: Mitogen-activated protein kinases. Crit Care Med. 30 1 Supp:S74–S79. 2002. View Article : Google Scholar
15	Ryu M, Kim EH, Chun M, Kang S, Shim B, Yu YB, Jeong G and Lee JS: Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk. J Ethnopharmacol. 115:184–193. 2008. View Article : Google Scholar : PubMed/NCBI
16	Tang J, Chen X, Tu W, Guo Y, Zhao Z, Xue Q, Lin C, Xiao J, Sun X, Tao T, et al: Propofol inhibits the activation of p38 through up-regulating the expression of annexin A1 to exert its anti-inflammation effect. PLoS One. 6:e278902011. View Article : Google Scholar : PubMed/NCBI
17	Barr RK and Bogoyevitch MA: The c-Jun N-terminal protein kinase family of mitogen-activated protein kinases (JNK MAPKs). Int J Biochem Cell Biol. 33:1047–1063. 2001. View Article : Google Scholar : PubMed/NCBI
18	Lin A: Activation of the JNK signaling pathway: Breaking the brake on apoptosis. Bioessays. 25:17–24. 2003. View Article : Google Scholar : PubMed/NCBI
19	Meng HY, Qu XB, Li N, Yuan S and Lin Z: Effects of pilose antler and antler glue on osteoporosis of ovariectomized rats. Zhong Yao Cai. 32:179–182. 2009.(In Chinese). PubMed/NCBI
20	Liu G, Ma C, Wang P, Zhang P, Qu X, Liu S, Zhai Z, Yu D, Gao J, Liang J, et al: Pilose antler peptide potentiates osteoblast differentiation and inhibits osteoclastogenesis via manipulating the NF-κB pathway. Biochem Biophys Res Commun. 491:388–395. 2017. View Article : Google Scholar : PubMed/NCBI
21	Ma C, Long H, Yang C, Cai W, Zhang T and Zhao W: Anti-inflammatory role of pilose antler peptide in LPS-induced lung injury. Inflammation. 40:904–912. 2017. View Article : Google Scholar : PubMed/NCBI
22	Chunhui Y, Wenjun C, Hui W, Liquan S, Changwei Z, Tianzhu Z and Wenhai Z: Pilose antler peptide protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling. Int J Biol Macromol. 99:15–20. 2017. View Article : Google Scholar : PubMed/NCBI
23	Suttie JM, Gluckman PD, Butler JH, Fennessy PF, Corson ID and Laas FJ: Insulin-like growth factor 1 (IGF-1) antler-stimulating hormone? Endocrinology. 116:846–848. 1985. View Article : Google Scholar : PubMed/NCBI
24	Wu T, Yang L, Chen Y, Ni Y, Jiang J, Zhang W, Zhou Q, Zheng X, Wang Q, Fu Z and Li H: Pilose antler polypeptides ameliorates hypoxic-ischemic encephalopathy by activated neurotrophic factors and SDF1/CXCR4 axis in rats. Acta Biochim Biophys Sin (Shanghai). 50:254–262. 2018. View Article : Google Scholar : PubMed/NCBI
25	Zhou QL, Guo YJ, Wang LJ, Wang Y, Liu YQ, Wang Y and Wang BX: Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing. Zhongguo Yao Li Xue Bao. 20:279–282. 1999.PubMed/NCBI
26	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
27	Shitaka Y, Matsuki N, Saito H and Katsuki H: Basic fibroblast growth factor increases functional L-type Ca2+ channels in fetal rat hippocampal neurons: Implications for neurite morphogenesis in vitro. J Neurosci. 16:6476–6489. 1996. View Article : Google Scholar : PubMed/NCBI
28	Facci L and Skaper SD: Culture of rodent cortical and hippocampal neurons. Methods Mol Biol. 846:49–56. 2012. View Article : Google Scholar : PubMed/NCBI
29	Lin JH, Deng LX, Wu ZY, Chen L and Zhang L: Pilose antler polypeptides promote chondrocyte proliferation via the tyrosine kinase signaling pathway. J Occup Med Toxicol. 6:272011. View Article : Google Scholar : PubMed/NCBI
30	Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X and Yan C: Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J Mol Cell Cardiol. 48:1225–1235. 2010. View Article : Google Scholar : PubMed/NCBI
31	Hui L, Bakiri L, Mairhorfer A, Schweifer N, Haslinger C, Kenner L, Komnenovic V, Scheuch H, Beug H and Wagner EF: p38alpha suppresses normal and cancer cell proliferation by antagonizing the JNK-c-Jun pathway. Nat Genet. 39:741–749. 2007. View Article : Google Scholar : PubMed/NCBI
32	Kostadinova R, Montagner A, Gouranton E, Fleury S, Guillou H, Dombrowicz D, Desreumaux P and Wahli W: GW501516-activated PPARβ/δ promotes liver fibrosis via p38-JNK MAPK-induced hepatic stellate cell proliferation. Cell Biosci. 2:342012. View Article : Google Scholar : PubMed/NCBI
33	Kuo WH, Chen JH, Lin HH, Chen BC, Hsu JD and Wang CJ: Induction of apoptosis in the lung tissue from rats exposed to cigarette smoke involves p38/JNK MAPK pathway. Chem Biol Interact. 155:31–42. 2005. View Article : Google Scholar : PubMed/NCBI