UBA2 promotes proliferation of colorectal cancer

He,Ping; Sun,Xun; Cheng,Hong‑Jing; Zou,Ya‑Bin; Wang,Quan; Zhou,Chang‑Li; Liu,Wan‑Qi; Hao,Yue‑Ming; Meng,Xiang‑Wei

doi:10.3892/mmr.2018.9613

UBA2 promotes proliferation of colorectal cancer

Authors:
- Ping He
- Xun Sun
- Hong‑Jing Cheng
- Ya‑Bin Zou
- Quan Wang
- Chang‑Li Zhou
- Wan‑Qi Liu
- Yue‑Ming Hao
- Xiang‑Wei Meng
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Affiliations: Department of Gastroenterology, Bethune First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Pathology, Bethune First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Gastrointestinal Surgery, Bethune First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: October 30, 2018 https://doi.org/10.3892/mmr.2018.9613
Pages: 5552-5562
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Small ubiquitin‑like modifier proteins are involved in tumorigenesis; however, the potential effects and functions of the family member ubiquitin‑like modifier‑activating enzyme 2 (UBA2) on colorectal cancer are not clear. The present study aimed to examine the effects of UBA2 on the proliferation of colorectal cancer cells in vitro and in vivo. The mRNA and protein expression levels of UBA2 in patients with colorectal cancer were measured by reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry, respectively. UBA2 expression levels in colorectal cancer tissues were significantly increased compared with the paracancerous normal tissues. The expression of UBA2 was also associated with higher stage colorectal cancer and poor prognosis. MTT and colony formation assays were used to examine proliferation in colorectal cancer cell lines. Flow cytometry was performed to examine the effects of UBA2 on the cell cycle and apoptosis of colorectal cancer cell lines and protein expression levels were examined by western blotting. Athymic nude mice were used to examine the ability of transfected colorectal cancer cells to form tumors in vivo. Downregulation of UBA2 inhibited the proliferation of colorectal cancer cell lines in vitro and in vivo through the regulation of cell cycle associated protein expression and apoptosis. Furthermore, downregulation of UBA2 decreased the expression levels of cyclin B1, B‑cell lymphoma-2, phosphorylated protein kinase B and E3 ubiquitin‑protein ligase MDM2 in colorectal cancer cells, whereas the expression levels of p21 and p27 were increased. UBA2 was demonstrated to serve an essential role in the proliferation of colorectal cancer and may be used as a potential biomarker to predict prognosis and as a therapeutic target in colorectal cancer.

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