Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2019 Volume 19 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2019 Volume 19 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells

  • Authors:
    • Shu‑Jun Liu
    • Wen‑Ting Wang
    • Feng‑Lan Zhang
    • Yong‑Hong Yu
    • Hua‑Jun Yu
    • Yan Liang
    • Ning Li
    • Yuan‑Bin Li
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1110-1116
    |
    Published online on: December 11, 2018
       https://doi.org/10.3892/mmr.2018.9738
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study investigated the effect of microRNA (miR)‑15a‑3p on the proliferation, migration and apoptosis of lens epithelial cells and its potential mechanism, in order to further elucidate the pathogenesis of age‑related cataracts (ARCs). The HLE‑B3 human lens epithelial cell line was transfected with miR‑15a‑3p mimic. Expression of the miR‑15a‑3p mimic was measured by fluorescence‑based reverse transcription‑quantitative polymerase chain reaction analysis. Cell proliferation, apoptosis, invasion and migration were investigated using MTT and plate clone formation assays, terminal deoxynucleotidyl transferase dUTP nick end labeling and flow cytometry, and a wound healing assay and Transwell assay, respectively. The protein expression levels of B‑cell lymphoma 2 (BCL2) and myeloid cell leukemia sequence 1 (MCL1) were also compared between transfected and wild‑type HLE‑B3 cells by western blot analysis. The results showed that transfection with the miR‑15a‑3p mimic significantly suppressed the proliferation of HLE‑B3 cells, induced cell apoptosis and increased the proportion of early apoptotic cells. The migration of HLE‑B3 cells was significantly inhibited following transfection with miR‑15a‑3p mimic (P<0.01), whereas cell invasion was unaffected (P>0.05). In addition, reduced protein levels of BCL2 and MCL1 were observed in the miR‑15a‑3p mimic‑transfected HLE‑B3 cells (P<0.01). In conclusion, miR‑15a‑3p may suppress cell proliferation and migration, and induce cell apoptosis in lens epithelial cells through inhibiting the expression of BCL2 and MCL1, which contributes to the onset of ARCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Ruotolo R, Grassi F, Percudani R, Rivetti C, Martorana D, Maraini G and Ottonello S: Gene expression profiling in human age-related nuclear cataract. Mol Vis. 9:538–548. 2003.PubMed/NCBI

2 

Li WC, Kuszak JR, Dunn K, Wang RR, Ma W, Wang GM, Spector A, Leib M, Cotliar AM and Weiss M: Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals. J Cell Biol. 130:169–181. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA. 102:13944–13949. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Calin GA, Cimmino A, Fabbri M, Ferracin M, Wojcik SE, Shimizu M, Taccioli C, Zanesi N, Garzon R, Aqeilan RI, et al: miR-15a and miR-16-1 cluster functions in human leukemia. Proc Natl Acad Sci USA. 105:5166–5171. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Aqeilan RI, Calin GA and Croce CM: miR-15a and miR-16-1 in cancer: Discovery, function and future perspectives. Cell Death Differ. 17:215–220. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Li Y, Liu S, Zhang F, Jiang P, Wu X and Liang Y: Expression of the microRNAs hsa-miR-15a and hsa-miR-16-1 in lens epithelial cells of patients with age-related cataract. Int J Clin Exp Med. 8:2405–2410. 2015.PubMed/NCBI

8 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Lin C, Wang J, Wang Y, Zhu P, Liu X, Li N, Liu J, Yu L and Wang W: GRP78 Participates in PCA3-regulated prostate cancer progression. Anticancer Res. 37:4303–4310. 2017.PubMed/NCBI

10 

West S: Epidemiology of cataract: Accomplishments over 25 years and future directions. Ophthalmic Epidemiol. 14:173–178. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Foster A and Resnikoff S: The impact of Vision 2020 on global blindness. Eye (Lond). 19:1133–1135. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Zhang F, Meng W and Tong B: Down-regulation of MicroRNA-133b suppresses apoptosis of lens epithelial cell by up-regulating BCL2L2 in age-related cataracts. Med Sci Monit. 22:4139–4145. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Dong Y, Zheng Y, Xiao J, Zhu C and Zhao M: MicroRNA let-7b induces lens epithelial cell apoptosis by targeting leucine-rich repeat containing G protein-coupled receptor 4 (Lgr4) in age-related cataract. Exp Eye Res. 147:98–104. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Peng CH, Liu JH, Woung LC, Lin TJ, Chiou SH, Tseng PC, Du WY, Cheng CK, Hu CC, Chien KH and Chen SJ: MicroRNAs and cataracts: Correlation among let-7 expression, age and the severity of lens opacity. Br J Ophthalmol. 96:747–751. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Wu C, Lin H, Wang Q, Chen W, Luo H, Chen W and Zhang H: Discrepant expression of microRNAs in transparent and cataractous human lenses. Invest Ophthalmol Vis Sci. 53:3906–3912. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Acunzo M and Croce CM: Downregulation of miR-15a and miR-16-1 at 13q14 in Chronic Lymphocytic Leukemia. Clin Chem. 62:655–656. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Renjie W and Haiqian L: miR-132, miR-15a and miR-16 synergistically inhibit pituitary tumor cell proliferation, invasion and migration by targeting Sox5. Cancer Lett. 356:568–578. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Huang E, Liu R and Chu Y: miRNA-15a/16: As tumor suppressors and more. Future Oncol. 11:2351–2363. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Lan F, Yue X, Ren G, Li H, Ping L, Wang Y and Xia T: miR-15a/16 enhances radiation sensitivity of non-small cell lung cancer cells by targeting the TLR1/NF-κB signaling pathway. Int J Radiat Oncol Biol Phys. 91:73–81. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Ye EA, Liu L, Jiang Y, Jan J, Gaddipati S, Suvas S and Steinle JJ: miR-15a/16 reduces retinal leukostasis through decreased pro-inflammatory signaling. J Neuroinflammation. 13:3052016. View Article : Google Scholar : PubMed/NCBI

21 

Cai CK, Zhao GY, Tian LY, Liu L, Yan K, Ma YL, Ji ZW, Li XX, Han K, Gao J, et al: miR-15a and miR-16-1 downregulate CCND1 and induce apoptosis and cell cycle arrest in osteosarcoma. Oncol Rep. 28:1764–1770. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Hao M, Zhang L, An G, Sui W, Yu Z, Zou D, Xu Y, Chang H and Qiu L: Suppressing miRNA-15a/-16 expression by interleukin-6 enhances drug-resistance in myeloma cells. J Hematol Oncol. 4:372011. View Article : Google Scholar : PubMed/NCBI

23 

Vogler M, Walter HS and Dyer MJS: Targeting anti-apoptotic BCL2 family proteins in haematological malignancies-from pathogenesis to treatment. Br J Haematol. 178:364–379. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Frenzel A, Grespi F, Chmelewskij W and Villunger A: Bcl2 family proteins in carcinogenesis and the treatment of cancer. Apoptosis. 14:584–596. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Tang J, Wang Z, Chen L, Huang G and Hu X: Gossypol acetate induced apoptosis of pituitary tumor cells by targeting the BCL-2 via the upregulated microRNA miR-15a. Int J Clin Exp Med. 8:9079–9085. 2015.PubMed/NCBI

26 

Xia L, Zhang D, Du R, Pan Y, Zhao L, Sun S, Hong L, Liu J and Fan D: miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer. 123:372–379. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu SJ, Wang WT, Zhang FL, Yu YH, Yu HJ, Liang Y, Li N and Li YB: miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells. Mol Med Rep 19: 1110-1116, 2019.
APA
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y. ... Li, Y. (2019). miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells. Molecular Medicine Reports, 19, 1110-1116. https://doi.org/10.3892/mmr.2018.9738
MLA
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y., Li, N., Li, Y."miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells". Molecular Medicine Reports 19.2 (2019): 1110-1116.
Chicago
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y., Li, N., Li, Y."miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells". Molecular Medicine Reports 19, no. 2 (2019): 1110-1116. https://doi.org/10.3892/mmr.2018.9738
Copy and paste a formatted citation
x
Spandidos Publications style
Liu SJ, Wang WT, Zhang FL, Yu YH, Yu HJ, Liang Y, Li N and Li YB: miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells. Mol Med Rep 19: 1110-1116, 2019.
APA
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y. ... Li, Y. (2019). miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells. Molecular Medicine Reports, 19, 1110-1116. https://doi.org/10.3892/mmr.2018.9738
MLA
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y., Li, N., Li, Y."miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells". Molecular Medicine Reports 19.2 (2019): 1110-1116.
Chicago
Liu, S., Wang, W., Zhang, F., Yu, Y., Yu, H., Liang, Y., Li, N., Li, Y."miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells". Molecular Medicine Reports 19, no. 2 (2019): 1110-1116. https://doi.org/10.3892/mmr.2018.9738
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team