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Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes

  • Authors:
    • Yang Wang
    • Hua‑Jun Sun
    • Rong‑Gui Li
    • Xiao‑Mei Wang
    • Zhi‑Qiang Cheng
    • Nan Lou
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518020, P.R. China, Department of Pathology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610015, P.R. China, Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, Jilin 130012, P.R. China, Department of Orthopaedics and Traumatology, The University of Hong Kong‑Shenzhen Hospital, Shenzhen, Guangdong 518053, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 967-973
    |
    Published online on: December 12, 2018
       https://doi.org/10.3892/mmr.2018.9753
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Abstract

Uncontrolled proliferation and defective apoptosis are two major factors responsible for maintaining the malignant properties of melanoma cells. Our previous study demonstrated that induced expression of four reprogramming factors remodeled the phenotype of B16‑F10 mouse melanoma cells into melanoma stem cells. The present study was conducted to investigate the effect of the four Yamanaka reprogramming factors, namely Oct4, Sox2, Klf4 and c‑Myc (OSKM), on the proliferation and apoptosis of melanoma cells, and to identify the responsible molecular signals. The results identified that expression of the four reprogramming factors was highly induced by doxycycline treatment in the stable melanoma cell clone that was transfected with a plasmid expressing these factors, driven by the Tet‑On element. It was further confirmed that induced expression of these factors enhanced the proliferation and suppressed the apoptosis of the melanoma cells. In addition, induced OSKM expression increased cell proliferation, accelerated the progression of the cell cycle, and upregulated the mRNA expression levels of Janus kinase 2 (JAK2) and Cyclin‑B1. Induced expression of these factors also decreased the apoptosis, as well as upregulated B‑cell lymphoma 2 (BCL‑2) and downregulated BCL‑2‑associated X (BAX) mRNA expression levels. Taken together, the results suggested that upregulated JAK2 and Cyclin‑B1 may be responsible for the enhanced proliferation of melanoma cells, and that BCL‑2 upregulation and BAX downregulation may account for the suppressed apoptosis of these cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Sun HJ, Li RG, Wang XM, Cheng ZQ and Lou N: Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Mol Med Rep 19: 967-973, 2019.
APA
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., & Lou, N. (2019). Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Molecular Medicine Reports, 19, 967-973. https://doi.org/10.3892/mmr.2018.9753
MLA
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., Lou, N."Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes". Molecular Medicine Reports 19.2 (2019): 967-973.
Chicago
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., Lou, N."Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes". Molecular Medicine Reports 19, no. 2 (2019): 967-973. https://doi.org/10.3892/mmr.2018.9753
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Sun HJ, Li RG, Wang XM, Cheng ZQ and Lou N: Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Mol Med Rep 19: 967-973, 2019.
APA
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., & Lou, N. (2019). Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Molecular Medicine Reports, 19, 967-973. https://doi.org/10.3892/mmr.2018.9753
MLA
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., Lou, N."Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes". Molecular Medicine Reports 19.2 (2019): 967-973.
Chicago
Wang, Y., Sun, H., Li, R., Wang, X., Cheng, Z., Lou, N."Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes". Molecular Medicine Reports 19, no. 2 (2019): 967-973. https://doi.org/10.3892/mmr.2018.9753
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