Open Access

Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells

  • Authors:
    • Jie Li
    • Min‑Hua Rong
    • Yi‑Wu Dang
    • Rong‑Quan He
    • Peng Lin
    • Hong Yang
    • Xiao‑Jiao Li
    • Dan‑Dan Xiong
    • Li‑Jie Zhang
    • Hui Qin
    • Cai‑Xia Feng
    • Xiao‑Yi Chen
    • Jin‑Cai Zhong
    • Jie Ma
    • Gang Chen
  • View Affiliations

  • Published online on: March 19, 2019     https://doi.org/10.3892/mmr.2019.10062
  • Pages: 4256-4270
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cinobufotalin is a chemical compound extracted from the skin of dried bufo toads that may have curative potential for certain malignancies through different mechanisms; however, these mechanisms remain unexplored in breast cancer. The aim of the present study was to investigate the antitumor mechanism of cinobufotalin in breast cancer by using microarray data and in silico analysis. The microarray data set GSE85871, in which cinobufotalin exerted influences on the MCF‑7 breast cancer cells, was acquired from the Gene Expression Omnibus database, and the differentially expressed genes (DEGs) were analyzed. Subsequently, protein interaction analysis was conducted, which clarified the clinical significance of core genes, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were used to analyze cinobufotalin‑related pathways. The Connectivity Map (CMAP) database was used to select existing compounds that exhibited curative properties similar to those of cinobufotalin. A total of 1,237 DEGs were identified from breast cancer cells that were treated with cinobufotalin. Two core genes, SRC proto‑oncogene non‑receptor tyrosine kinase and cyclin‑dependent kinase inhibitor 2A, were identified as serving a vital role in the onset and development of breast cancer, and their expression levels were markedly reduced following cinobufotalin treatment as detected by the microarray of GSE85871. It also was revealed that the ‘neuroactive ligand‑receptor interaction’ and ‘calcium signaling’ pathways may be crucial for cinobufotalin to perform its functions in breast cancer. Conducting a matching search in CMAP, miconazole and cinobufotalin were indicated to possessed similar molecular mechanisms. In conclusion, cinobufotalin may serve as an effective compound for the treatment of a subtype of breast cancer that is triple positive for the presence of estrogen, progesterone and human epidermal growth factor receptor‑2 receptors, and its mechanism may be related to different pathways. In addition, cinobufotalin is likely to exert its antitumor influences in a similar way as miconazole in MCF‑7 cells.
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May-2019
Volume 19 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Li J, Rong MH, Dang YW, He RQ, Lin P, Yang H, Li XJ, Xiong DD, Zhang LJ, Qin H, Qin H, et al: Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells. Mol Med Rep 19: 4256-4270, 2019.
APA
Li, J., Rong, M., Dang, Y., He, R., Lin, P., Yang, H. ... Chen, G. (2019). Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells. Molecular Medicine Reports, 19, 4256-4270. https://doi.org/10.3892/mmr.2019.10062
MLA
Li, J., Rong, M., Dang, Y., He, R., Lin, P., Yang, H., Li, X., Xiong, D., Zhang, L., Qin, H., Feng, C., Chen, X., Zhong, J., Ma, J., Chen, G."Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells". Molecular Medicine Reports 19.5 (2019): 4256-4270.
Chicago
Li, J., Rong, M., Dang, Y., He, R., Lin, P., Yang, H., Li, X., Xiong, D., Zhang, L., Qin, H., Feng, C., Chen, X., Zhong, J., Ma, J., Chen, G."Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells". Molecular Medicine Reports 19, no. 5 (2019): 4256-4270. https://doi.org/10.3892/mmr.2019.10062