miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN

Zhang,Si; He,Kang; Zhou,Weiwei; Cao,Jun; Jin,Zuolin

doi:10.3892/mmr.2019.10083

miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN

Authors:
- Si Zhang
- Kang He
- Weiwei Zhou
- Jun Cao
- Zuolin Jin
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Affiliations: State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
Published online on: March 26, 2019 https://doi.org/10.3892/mmr.2019.10083
Pages: 4288-4296
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) serve important roles in regulating inflammatory responses at the post‑transcriptional level. In the present study, the limma package was used to analyze the GSE43300 array dataset downloaded from the Gene Expression Omnibus database. It was identified that several miRNAs, including miR‑494‑3p, were upregulated in lipopolysaccharide (LPS)‑treated RAW264.7 macrophages compared to control cells. Transfection experiments indicated that overexpressing miR‑494‑3p inhibited production of LPS‑induced proinflammatory cytokines, including interleukin‑1β and tumor necrosis factor‑α. Conversely, knockdown of miR‑494‑3p enhanced cytokine expression. Bioinformatics prediction and luciferase assay both revealed that miR‑494‑3p could directly target phosphatase and tensin homolog (PTEN) and upregulate protein kinase B activity. In addition, miR‑494‑3p mimics suppressed p65 translocation to the nucleus. Similar effects were observed following PTEN silencing. In conclusion, the results of the present study revealed that miR‑494‑3p may act as an important immune regulator in LPS‑stimulated macrophages, and be an effective therapeutic target for treating infections in the future.

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