Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

Zhao,Qianwen; Yang,Jie; Liu,Baolin; Huang,Fang; Li,Yuehua

doi:10.3892/mmr.2019.10127

Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

Authors:
- Qianwen Zhao
- Jie Yang
- Baolin Liu
- Fang Huang
- Yuehua Li
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Affiliations: Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of Pharmacology of Chinese Materia Medic, China Pharmaceutical University, Nanjing, Jiangsu 211198, P.R. China
Published online on: April 4, 2019 https://doi.org/10.3892/mmr.2019.10127
Pages: 4797-4805
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Perivascular adipose tissue (PVAT) is considered to serve a vital role during the development of endothelial dysfunction. The current study investigated the effect of exosomes derived from mangiferin‑stimulated PVAT on endothelial function, including regeneration, migration, apoptosis and inflammation. The number of exosomes secreted by PVAT was increased by stimulation with mangiferin (0.1, 1 or 10 µM), and uptake of these exosomes by endothelial cells was observed. Exosomes produced by stimulation of PVAT with mangiferin reversed the effects of inflammation‑induced endothelial dysfunction following palmitic acid (PA) treatment. Furthermore, nuclear factor (NF)‑κB signaling in endothelial cells was significantly increased when treated with PA‑induced PVAT‑derived exosomes, whereas exosomes from the supernatant of PVAT stimulated with mangiferin reduced p65 and p50 phosphorylation levels in the cells, and inhibited p65 transportation to the nucleus. Taken together, the present study demonstrated that exosomes derived from mangiferin‑stimulated PVAT supernatant inhibited inflammation‑induced endothelial dysfunction via modulation of NF‑κB signaling.

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