Synergistic protection of matrine and lycopene against lipopolysaccharide‑induced acute lung injury in mice

Li,Wu‑Wei; Wang,Tao‑Yuan; Cao,Bo; Liu,Bin; Rong,Yu‑Mei; Wang,Juan‑Juan; Wei,Fei; Wei,Lu‑Qing; Chen,Hong; Liu,Yan‑Xia

doi:10.3892/mmr.2019.10278

Synergistic protection of matrine and lycopene against lipopolysaccharide‑induced acute lung injury in mice

Authors:
- Wu‑Wei Li
- Tao‑Yuan Wang
- Bo Cao
- Bin Liu
- Yu‑Mei Rong
- Juan‑Juan Wang
- Fei Wei
- Lu‑Qing Wei
- Hong Chen
- Yan‑Xia Liu
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Affiliations: Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin 300162, P.R. China, Department of Health Service, Logistics University of Chinese People's Armed Police Force, Tianjin 300300, P.R. China
Published online on: May 22, 2019 https://doi.org/10.3892/mmr.2019.10278
Pages: 455-462
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute lung injury (ALI) is a major cause of morbidity and mortality globally, and is characterized by widespread inflammation in the lungs. Increased production of reactive oxygen species is hypothesized to be associated with ALI. Matrine and lycopene are active products present in traditional Chinese medicine. Matrine is an effective inhibitor of inflammation, whereas lycopene decreases lipid peroxidation. Therefore, it was hypothesized that combinatorial treatment with matrine and lycopene may provide synergistic protection against ALI. In the present study, mice were treated with dexamethasone (DEX; 5 mg/kg), matrine (25 mg/kg), lycopene (100 mg/kg), and matrine (25 mg/kg) + lycopene (100 mg/kg) for 7 days prior to injury induction using lipopolysaccharide (LPS; 5 mg/kg) for 6 h. Lung tissues were collected following the sacrifice of the mice and hematoxylin and eosin staining was used for histological analysis. Malondialdehyde (MDA), glutathione (GSH) and myeloperoxidas (MPO) levels were examined by respective kits. The expressions of interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) were evaluated by ELISA. The expressions of IκBα and NF‑κB p65 were examined by reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemistry. The results indicated that the combined treatment exhibited a similar effect to DEX, both of which attenuated lung structural injuries, downregulated the expressions of IL‑6, TNF‑α, MPO and MDA, and upregulated that of GSH. Furthermore, the combined treatment and DEX inhibited NF‑κB p65 activation. The present study revealed that combined treatment with matrine and lycopene exhibited protective effects on an LPS‑induced mouse model of ALI, suggesting that they may serve as a potential alternative to glucocorticoid therapy for ALI.

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