PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway

Liu,Shen; Jia,Jun; Zhou,Hengxing; Zhang,Chi; Liu,Lu; Liu,Jun; Lu,Lu; Li,Xueying; Kang,Yi; Lou,Yongfu; Cai,Zhiwei; Ren,Yiming; Kong,Xiaohong; Feng,Shiqing

doi:10.3892/mmr.2019.10670

PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway

Authors:
- Shen Liu
- Jun Jia
- Hengxing Zhou
- Chi Zhang
- Lu Liu
- Jun Liu
- Lu Lu
- Xueying Li
- Yi Kang
- Yongfu Lou
- Zhiwei Cai
- Yiming Ren
- Xiaohong Kong
- Shiqing Feng
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Affiliations: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Trauma Orthopedics, Tianjin Hospital, Tianjin 300211, P.R. China, Key Laboratory of Immuno Microenvironment and Disease of the Educational Ministry of China, Department of Immunology, Tianjin Medical University, Tianjin 300070, P.R. China, Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, P.R. China
Published online on: September 10, 2019 https://doi.org/10.3892/mmr.2019.10670
Pages: 4059-4066

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Abstract

The present study aimed to explore the role of the PTEN/Akt/mTOR signaling pathway in the neurite outgrowth and apoptosis of cortical neurons. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of the neurites were calculated. Immunohistochemical staining and TUNEL data were analyzed. Neurites treated with PTEN inhibitor exhibited significant enhancements in elongation, initiation and crossing abilities when they encountered chondroitin sulfate proteoglycans in vitro. These effects disappeared when the PTEN/Akt/mTOR signaling pathway was blocked. Neurons exhibited significant enhancements in survival ability following PTEN inhibition. The present study demonstrated that PTEN inhibition can promote axonal elongation and initiation in cerebral cortical neurons, as well as the ability to cross the chondroitin sulfate proteoglycan border. In addition, PTEN inhibition is useful for protecting the neuron from apoptosis. The PTEN/Akt/mTOR signaling pathway is an important signaling pathway.

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