Open Access

Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats

  • Authors:
    • Yusuke Wakasa
    • Norihisa Kimura
    • Toshiyuki Yamada
    • Takeshi Shimizu
    • Kenichi Hakamada
    • Shigeki Tsuchida
  • View Affiliations

  • Published online on: September 13, 2019     https://doi.org/10.3892/mmr.2019.10681
  • Pages: 4367-4375
  • Copyright: © Wakasa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Infection is a frequent complication of liver transplantation or partial hepatectomy (PH) and sometimes results in cholestasis. We examined factors involved in infection‑induced cholestasis after PH, employing a rat PH model and lipopolysaccharide (LPS) as a bacterial toxin. Male Sprague‑Dawley rats were subjected to 70% PH and/or LPS injection, and tissues were harvested at 0, 24, 72 and 168 h. Gene expression was analyzed by microarray analysis and reverse transcription‑quantitative polymerase chain reaction, and protein levels and localization were analyzed by western blotting and immunohistochemistry, respectively. Plasma bile acid levels were significantly higher in the LPS + PH group than in the PH group. Ribonucleotide reductase regulatory subunit M2 and proliferating cell nuclear antigen peaked at 24 and 72 h in the PH group and LPS + PH group, respectively, indicating a delay in cell proliferation in the latter group. The sodium‑dependent taurocholate cotransporting polypeptide and organic‑anion‑transporting polypeptide 1a1 and 1a2 were reduced in the PH group at 24 h, and were not further decreased in the LPS + PH group. Chemokine ligand 9 (Cxcl9), a chemokine involved in M2 macrophage polarization, increased after 24 h in the LPS and the LPS + PH groups. The number and shape of Cxcl9‑positive cells were similar to CD163‑positive cells, suggesting that such cells produced the chemokine. Hematopoietic prostaglandin D2 synthase (Ptgds2) was only detected in hepatocytes of the LPS + PH group exhibiting a delay in cell proliferation. Thus, Kupffer cells activated with LPS were suggested to be responsible for a delay in hepatocyte proliferation after PH.
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November-2019
Volume 20 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wakasa Y, Kimura N, Yamada T, Shimizu T, Hakamada K and Tsuchida S: Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats. Mol Med Rep 20: 4367-4375, 2019.
APA
Wakasa, Y., Kimura, N., Yamada, T., Shimizu, T., Hakamada, K., & Tsuchida, S. (2019). Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats. Molecular Medicine Reports, 20, 4367-4375. https://doi.org/10.3892/mmr.2019.10681
MLA
Wakasa, Y., Kimura, N., Yamada, T., Shimizu, T., Hakamada, K., Tsuchida, S."Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats". Molecular Medicine Reports 20.5 (2019): 4367-4375.
Chicago
Wakasa, Y., Kimura, N., Yamada, T., Shimizu, T., Hakamada, K., Tsuchida, S."Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats". Molecular Medicine Reports 20, no. 5 (2019): 4367-4375. https://doi.org/10.3892/mmr.2019.10681