Hydrogen sulfide protects against high glucose‑induced lipid metabolic disturbances in 3T3‑L1 adipocytes via the AMPK signaling pathway

Pan,Zhe; Wang,Jue; Xu,Min; Chen,Shihong; Li,Xiaobo; Sun,Aili; Lou,Nengjun; Ni,Yihong

doi:10.3892/mmr.2019.10685

Hydrogen sulfide protects against high glucose‑induced lipid metabolic disturbances in 3T3‑L1 adipocytes via the AMPK signaling pathway

Authors:
- Zhe Pan
- Jue Wang
- Min Xu
- Shihong Chen
- Xiaobo Li
- Aili Sun
- Nengjun Lou
- Yihong Ni
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Affiliations: Department of Endocrinology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Central Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
Published online on: September 16, 2019 https://doi.org/10.3892/mmr.2019.10685
Pages: 4119-4124
Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant lipid metabolism contributes to the development of type 2 diabetes mellitus. The mechanisms by which hydrogen sulfide (H2S), an endogenous gasotransmitter, regulates lipid metabolism remain unclear. The aim of the present study was to investigate if the protective effects of H2S during high glucose (HG)‑induced lipid accumulation in 3T3‑L1 adipocytes may be mediated by AMP‑activated protein kinase (AMPK). Triglyceride (TG) content and the production of H2S were determined using adipogenesis colorimetric assay kits and H2S synthesis methods. The levels of monocyte chemoattractant protein‑1 and adiponectin were evaluated by ELISA. Total AMPK and phosphorylated AMPK levels were assessed by western blot analysis. HG increased the cellular level of TG and decreased H2S production in 3T3‑L1 adipocytes. The H2S donor, sodium hydrosulfide (NaHS) protected against the HG‑induced accumulation of TG in 3T3‑L1 adipocytes. Furthermore, NaHS suppressed HG‑induced TG accumulation by activating AMPK. Collectively, the findings of the present study suggested that HG induced lipid accumulation in 3T3‑L1 adipocytes, and AMPK activation may underlie the lipid‑lowering effects of H2S.

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