Effects of the transcription factor Olig1 on the differentiation and remyelination of oligodendrocyte precursor cells after focal cerebral ischemia in rats

Zhao,Hong; Gao,Xiao‑Yu; Liu,Zan‑Hua; Lin,Jian‑Wen; Wang,Su‑Ping; Wang,De‑Xin; Zhang,Yong‑Bo

doi:10.3892/mmr.2019.10713

Effects of the transcription factor Olig1 on the differentiation and remyelination of oligodendrocyte precursor cells after focal cerebral ischemia in rats

Authors:
- Hong Zhao
- Xiao‑Yu Gao
- Zan‑Hua Liu
- Jian‑Wen Lin
- Su‑Ping Wang
- De‑Xin Wang
- Yong‑Bo Zhang
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Affiliations: Department of Neurology, Dalian Municipal Central Hospital, Dalian, Liaoning 116033, P.R. China, Department of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Neurology, Nanjing Gaochun People's Hospital, Nanjing, Jiangsu 211300, P.R. China, Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
Published online on: September 26, 2019 https://doi.org/10.3892/mmr.2019.10713
Pages: 4603-4611
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The differentiation and maturation of oligodendrocyte precursor cells (OPCs) is important for remyelination in the central nervous system. Nevertheless, this process is often limited and incomplete in ischemic injury. Oligodendrocyte transcription factor 1 (Olig1) is important for the maturation of OPCs and the repair of demyelinated lesions. However, how Olig1 modulates the development of OPCs or the remyelination associated with ischemic injury remains unclear. The present study aimed to examine alterations in OPCs, and the expression of myelin and Olig1, at different time-points after focal cerebral ischemia using immunohistochemistry and western blot techniques to elucidate the role of Olig1 in the maturation of OPCs and remyelination. The present results showed that the expression of Olig1 significantly decreased at 1 day after middle cerebral artery occlusion (MCAO) and returned to normal levels from day 3 to 28. Additionally, Olig1 was found to translocate into the nucleus following ischemia in the brain. The number of OPCs in the ischemic striatum significantly declined at days 1 and 3 following MCAO, and increased at days 7, 14 and 28 compared with the control. The expression of myelin basic protein, a marker of mature oligodendrocytes and myelin, gradually decreased from day 1 to 7 after ischemia and recovered at day 14 and 28; however, the levels were lower than those in the control group. The present results indicated that the restored normal level of Olig1 following ischemia may play an important role in the maturation of OPCs through its translocation into the nucleus, where it may promote the growth and development of myelin under pathological conditions. However, this endogenous recovery mechanism fails to fully repair the demyelinated lesion. The data of the present study may help clinicians understand the expression pattern of Olig1 and its potential role in endogenous remyelination after ischemia, which may have implications for the treatment of diseases that lead to demyelination.

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