Open Access

Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway

  • Authors:
    • Shirui Tan
    • Jian Xu
    • Aiyun Lai
    • Ruomei Cui
    • Ru Bai
    • Shu Li
    • Wei Liang
    • Guofang Zhang
    • Shaoquan Jiang
    • Shuang Liu
    • Mai Zheng
    • Wei Wang
  • View Affiliations

  • Published online on: January 14, 2019     https://doi.org/10.3892/mmr.2019.9854
  • Pages: 2057-2064
  • Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the anti‑arthritic effects of curculigoside isolated from the rhizome of Curculigo orchioides Gaertn in vivo and in vitro, as well as to determine the potential underlying mechanisms. A rat model of arthritis was induced with type II collagen. Arthritic rats were treated with curculigoside (50 mg/kg) and blood samples were collected to determine serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6, IL‑10, IL‑12 and IL‑17A. Furthermore, indices of the thymus and spleen were determined. The anti‑proliferative effects of curculigoside were detected with Cell Counting kit‑8 assays in rheumatoid arthritis‑derived fibroblast‑like synoviocyte MH7A cells. In addition, expression levels of Janus kinase (JAK)1, JAK3, signal transducer and activator of transcription (STAT)3, nuclear factor (NF)‑κB p65 and its inhibitor (IκB) were determined by western blotting. The results revealed that curculigoside inhibited paw swelling and arthritis scores in type II collagen‑induced arthritic (CIA) rats. Additionally, curculigoside decreased serum levels of TNF‑α, IL‑1β, IL‑6, IL‑10, IL‑12 and IL‑17A in CIA rats. Curculigoside also significantly inhibited MH7A cell proliferation in a time and concentration‑dependent manner. Furthermore, treatment downregulated the expression of JAK1, JAK3 and STAT3, and upregulated cytosolic nuclear factor (NF)‑κB p65 and IκB. In conclusion, the results of the present study indicated that curculigoside exhibited significant anti‑arthritic effects in vivo and in vitro, and the molecular mechanism may be associated with the JAK/STAT/NF‑κB signaling pathway.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Tan S, Xu J, Lai A, Cui R, Bai R, Li S, Liang W, Zhang G, Jiang S, Liu S, Liu S, et al: Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway. Mol Med Rep 19: 2057-2064, 2019.
APA
Tan, S., Xu, J., Lai, A., Cui, R., Bai, R., Li, S. ... Wang, W. (2019). Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway. Molecular Medicine Reports, 19, 2057-2064. https://doi.org/10.3892/mmr.2019.9854
MLA
Tan, S., Xu, J., Lai, A., Cui, R., Bai, R., Li, S., Liang, W., Zhang, G., Jiang, S., Liu, S., Zheng, M., Wang, W."Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway". Molecular Medicine Reports 19.3 (2019): 2057-2064.
Chicago
Tan, S., Xu, J., Lai, A., Cui, R., Bai, R., Li, S., Liang, W., Zhang, G., Jiang, S., Liu, S., Zheng, M., Wang, W."Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway". Molecular Medicine Reports 19, no. 3 (2019): 2057-2064. https://doi.org/10.3892/mmr.2019.9854