MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12

Dai,Yankun; Liu,Shanglun; Xie,Xueguan; Ding,Mingsheng; Zhou,Quan; Zhou,Xiaoqing

doi:10.3892/mmr.2019.9859

MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12

Authors:
- Yankun Dai
- Shanglun Liu
- Xueguan Xie
- Mingsheng Ding
- Quan Zhou
- Xiaoqing Zhou
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Affiliations: Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, P.R. China, Department of Orthopedics, Huai'an Hospital of Traditional Chinese Medicine, Huai'an, Jiangsu 223200, P.R. China
Published online on: January 15, 2019 https://doi.org/10.3892/mmr.2019.9859
Pages: 2231-2237
Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the biological function and underlying molecular mechanisms of miR-31 in osteoarthritis (OA). Reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑31 expression, and it was found that miR‑31 was downregulated in the cartilage tissues of OA patients. microRNA.org was used to predict the gene targets of miR‑31, and dual luciferase reporter assays were used to verify that C‑X‑C motif chemokine ligand 12 (CXCL12) was a direct target of miR‑31. The human chondrocyte cell line CHON‑001 was used to perform MTT and cell migration assays. Western blotting was used to measure the protein expression of CXCL12, type I collagen and aggrecan. The results suggested that CXCL12 was a target of miR‑31, and the expression of CXCL12 was negatively regulated by miR‑31 in CHON‑001 cells. miR‑31 increased CHON‑001 cell viability and migration, as well as the expression of type I collagen and aggrecan. Furthermore, the overexpression of CXCL12 eliminated the effects of miR‑31 mimics on CHON‑001 cells. In conclusion, the data indicated that miR‑31 promoted chondrocyte viability and migration by directly targeting CXCL12, which provided evidence for CXCL12 as a potential target in OA therapy.

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1	Redman SN, Oldfield SF and Archer CW: Current strategies for articular cartilage repair. Eur Cell Mater. 9:23–32. 2005. View Article : Google Scholar : PubMed/NCBI
2	Tsezou A: Osteoarthritis year in review 2014: Genetics and genomics. Osteoarthritis Cartilage. 22:2017–2024. 2014. View Article : Google Scholar : PubMed/NCBI
3	Tang X, Wang S, Zhang Y, Niu J, Tao K, Zhang Y and Lin J: The prevalence of symptomatic knee osteoarthritis in China: Results from China health and retirement longitudinal study. Arthritis Rheumatol. 68:648–653. 2016. View Article : Google Scholar : PubMed/NCBI
4	Dowthwaite GP, Bishop JC, Redman SN, Khan IM, Rooney P, Evans DJ, Haughton L, Bayram Z, Boyer S, Thomson B, et al: The surface of articular cartilage contains a progenitor cell population. J Cell Sci. 117:889–897. 2004. View Article : Google Scholar : PubMed/NCBI
5	Xian CJ and Foster BK: Repair of injured articular and growth plate cartilage using mesenchymal stem cells and chondrogenic gene therapy. Curr Stem Cell Res Ther. 1:213–229. 2006. View Article : Google Scholar : PubMed/NCBI
6	Ji YH, Ji JL, Sun FY, Zeng YY, He XH, Zhao JX, Yu Y, Yu SH and Wu W: Quantitative proteomics analysis of chondrogenic differentiation of C3H10T1/2 mesenchymal stem cells by iTRAQ labeling coupled with on-line two-dimensional LC/MS/MS. Mol Cell Proteomics. 9:550–564. 2010. View Article : Google Scholar : PubMed/NCBI
7	Fox JM, Chamberlain G, Ashton BA and Middleton J: Recent advances into the understanding of mesenchymal stem cell trafficking. Br J Haematol. 137:491–502. 2007. View Article : Google Scholar : PubMed/NCBI
8	Marquez-Curtis LA and Janowska-Wieczorek A: Enhancing the migration ability of mesenchymal stromal cells by targeting the SDF-1/CXCR4 axis. Biomed Res Int 2013. 5610982013.
9	Li Q, Guo Y, Chen F, Liu J and Jin P: Stromal cell derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing. Exp Ther Med. 12:45–50. 2016. View Article : Google Scholar : PubMed/NCBI
10	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
11	Wei F, Moore DC, Wei L, Li Y, Zhang G, Wei X, Lee JK and Chen Q: Attenuation of osteoarthritis via blockade of the SDF-1/CXCR4 signaling pathway. Arthritis Res Ther. 14:R1772012. View Article : Google Scholar : PubMed/NCBI
12	Lisignoli G, Toneguzzi S, Piacentini A, Cristino S, Grassi F, Cavallo C and Facchini A: CXCL12 (SDF-1) and CXCL13 (BCA-1) chemokines significantly induce proliferation and collagen type I expression in osteoblasts from osteoarthritis patients. J Cell Physiol. 206:78–85. 2006. View Article : Google Scholar : PubMed/NCBI
13	Maroudas AI: Balance between swelling pressure and collagen tension in normal and degenerate cartilage. Nature. 260:808–809. 1976. View Article : Google Scholar : PubMed/NCBI
14	Chou MC, Tsai PH, Huang GS, Lee HS, Lee CH, Lin MH, Lin CY and Chung HW: Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection. Osteoarthritis Cartilage. 17:441–447. 2009. View Article : Google Scholar : PubMed/NCBI
15	Iliopoulos D, Malizos KN, Oikonomou P and Tsezou A: Integrative microRNA and proteomic approsteoarthritisches identify novel osteoarthritis genes and their collaborative metabolic and inflammatory networks. PLoS One. 3:e37402008. View Article : Google Scholar : PubMed/NCBI
16	Sumiyoshi K, Kubota S, Ohgawara T, Kawata K, Nishida T, Shimo T, Yamashiro T and Takigawa M: Identification of miR-1 as a micro RNA that supports late-stage differentiation of growth cartilage cells. Biochem Biophys Res Commun. 402:286–290. 2010. View Article : Google Scholar : PubMed/NCBI
17	Zhang W, Cheng P, Hu W, Yin W, Guo F, Chen A and Huang H: Inhibition of microRNA-384-5p alleviates osteoarthritis through its effects on inhibiting apoptosis of cartilage cells via the NF-κB signaling pathway by targeting SOX9. Cancer Gene Ther. 25:326–338. 2018. View Article : Google Scholar : PubMed/NCBI
18	Zhang W, Hsu P, Zhong B, Guo S and Zhang C, Wang Y, Luo C, Zhan Y and Zhang C: MiR-34a enhances chondrocyte apoptosis, senescence and facilitates development of osteoarthritis by targeting DLL1 and regulating PI3K/AKT pathway. Cell Physiol Biochem. 48:1304–1316. 2018. View Article : Google Scholar : PubMed/NCBI
19	Wu J, Zou M, Ping A, Deng Z and Cai L: MicroRNA-449a upregulation promotes chondrocyte extracellular matrix degradation in osteoarthritis. Biomed Pharmacother. 105:940–946. 2018. View Article : Google Scholar : PubMed/NCBI
20	Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P and Fan X: Effects of miR-31 on the osteogenesis of human mesenchymal stem cells. Biochem Biophys Res Commun. 446:98–104. 2014. View Article : Google Scholar : PubMed/NCBI
21	Deng Y, Wu S, Zhou H, Bi X, Wang Y, Hu Y, Gu P and Fan X: Effects of a miR-31, Runx2, and Satb2 regulatory loop on the osteogenic differentiation of bone mesenchymal stem cells. Stem Cells Dev. 22:2278–2286. 2013. View Article : Google Scholar : PubMed/NCBI
22	Liu Y, Wang Y, He X, Zhang S, Wang K, Wu H and Chen L: LncRNA TINCR/miR-31-5p/C/EBP-α feedback loop modulates the adipogenic differentiation process in human adipose tissue-derived mesenchymal stem cells. Stem Cell Res. 32:35–42. 2018. View Article : Google Scholar : PubMed/NCBI
23	Lu W, Shi J, Zhang J, Lv Z, Guo F, Huang H, Zhu W and Chen A: CXCL12/CXCR4 axis regulates aggrecanase activation and cartilage degradation in a post-traumatic osteoarthritis rat model. Int J Mol Sci. 17:E15222016. View Article : Google Scholar : PubMed/NCBI
24	Dong Y, Liu H, Zhang X, Xu F, Qin L, Cheng P, Huang H, Guo F, Yang Q and Chen A: Inhibition of CXCL12/CXCR4 signalling in subchondral bone attenuates post-traumatic osteoarthritis. Int J Mol Sci. 17:E9432016. View Article : Google Scholar : PubMed/NCBI
25	He W, Wang M, Wang Y, Wang Q and Luo B: Plasma and synovial fluid CXCL12 levels are correlated with disease severity in patients with knee osteoarthritis. J Arthroplasty. 31:373–377. 2016. View Article : Google Scholar : PubMed/NCBI