Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice

Li,Li; Xu,Xiaoyi; Wu,Leilei; Zhu,Haicheng; He,Zhipeng; Zhang,Bo; Chi,Yanjun; Song,Gaochen

doi:10.3892/mmr.2019.9862

Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice

Authors:
- Li Li
- Xiaoyi Xu
- Leilei Wu
- Haicheng Zhu
- Zhipeng He
- Bo Zhang
- Yanjun Chi
- Gaochen Song
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Affiliations: Department of Basic Medicine, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Digestive Surgery, Mudanjiang Anorectal Hospital, Mudanjiang, Heilongjiang 157000, P.R. China, Department of Clinical Laboratory, Tumor Hospital of Mudanjiang City, Mudanjiang, Heilongjiang 157009, P.R. China, Department of Brain Surgery, Mudanjiang First People's Hospital, Mudanjiang, Heilongjiang 157011, P.R. China
Published online on: January 15, 2019 https://doi.org/10.3892/mmr.2019.9862
Pages: 2254-2262
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was ~1 g (~7‑10 days after inoculation). The sera proteins from each group were then collected and subjected to two‑dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA‑binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma‑bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma‑bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling.

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