miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway

  • Authors:
    • Hong Wei
    • Ruifeng Liu
    • Xvli Guo
    • Yin Zhou
    • Bo Sun
    • Jialin Wang
  • View Affiliations

  • Published online on: January 22, 2019     https://doi.org/10.3892/mmr.2019.9885
  • Pages: 2361-2367
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Abstract

The present study investigated the role of microRNA‑135a (miR‑135a) in cutaneous T‑cell lymphoma (CTCL) proliferation. Compared with the normal T lymphocyte control cell line, the mRNA and protein levels of GATA binding protein 3 (GATA‑3) were markedly increased in the Hut78 cell line and miR‑135a was markedly decreased (P<0.05). Based on bioinformatics, the target gene of miR‑135a was identified as GATA‑3. Dual luciferase and pre‑miR‑135a assays showed that miR‑135a regulated the translation of GATA‑3. In addition, the overexpression of miR‑135a mimics decreased the protein levels of GATA‑3 and thymocyte selection‑associated high mobility group box (TOX). The substantially increased mRNA and protein levels of GATA‑3 may be associated with the downregulation of miR‑135a, leading to T‑cell deregulation and proliferation through GATA‑3/TOX regulation and subsequently causing CTCL.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wei H, Liu R, Guo X, Zhou Y, Sun B and Wang J: miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway. Mol Med Rep 19: 2361-2367, 2019.
APA
Wei, H., Liu, R., Guo, X., Zhou, Y., Sun, B., & Wang, J. (2019). miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway. Molecular Medicine Reports, 19, 2361-2367. https://doi.org/10.3892/mmr.2019.9885
MLA
Wei, H., Liu, R., Guo, X., Zhou, Y., Sun, B., Wang, J."miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway". Molecular Medicine Reports 19.3 (2019): 2361-2367.
Chicago
Wei, H., Liu, R., Guo, X., Zhou, Y., Sun, B., Wang, J."miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway". Molecular Medicine Reports 19, no. 3 (2019): 2361-2367. https://doi.org/10.3892/mmr.2019.9885