High expression of CDCA7 predicts tumor progression and poor prognosis in human colorectal cancer

Li,Siman; Huang,Jiean; Qin,Mengbin; Zhang,Jinxiu; Liao,Cun

doi:10.3892/mmr.2020.11089

High expression of CDCA7 predicts tumor progression and poor prognosis in human colorectal cancer

Authors:
- Siman Li
- Jiean Huang
- Mengbin Qin
- Jinxiu Zhang
- Cun Liao
View Affiliations

Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530007, P.R. China, Department of Colorectal‑Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: April 22, 2020 https://doi.org/10.3892/mmr.2020.11089
Pages: 57-66
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Colorectal cancer (CRC) is one of the most fatal types of cancer worldwide. This study aimed to determine the predictive and prognostic values of cell division cycle associated protein 7 (CDCA7) in CRC. Firstly, the relationship between CDCA7 and CRC was assessed through bioinformatics analysis. Subsequently, CDCA7 expression levels were detected in various CRC cell lines, as well as 15 fresh human CRC tissues and their paired adjacent normal colorectal tissues using reverse transcription‑quantitative PCR and western blotting. Additionally, immunohistochemical staining was used to determine the levels of CDCA7 in 104 CRC tissues and their paired adjacent normal colorectal tissues. The present study revealed that CDCA7 expression was upregulated in CRC tissues and cell lines. The positive expression rates of CDCA7 in normal and CRC tissues were 26.92 and 75.96%, respectively. The intensities of CDCA7 immunostaining were significantly associated with CRC invasion depth, lymph node metastasis, tumor‑node‑metastasis stage and distant metastasis. However, no significant differences in sex, age, tumor size and CRC differentiation were found between high and low CDCA7 expression groups. Furthermore, patients with low CDCA7 expression exhibited a greater overall survival rate of CRC compared to those with high CDCA7 expression. The findings of this study indicated that CDCA7 may serve a significant role in CRC prognosis and progression, and may be considered a novel biomarker for the prediction of patient survival after colectomy.

View Figures

View References

1	Torre LA, Freddie B, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
2	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI
3	Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI
4	Chiu HM, Hsu WF, Chang LC and Wu MH: Colorectal cancer screening in Asia. Curr Gastroenterol Rep. 19:472017. View Article : Google Scholar : PubMed/NCBI
5	Calon A, Espinet E, Palomo-Ponce S, Tauriello DV, Iglesias M, Céspedes MV, Sevillano M, Nadal C, Jung P, Zhang XH, et al: Dependency of colorectal cancer on a TGF-β-driven program in stromal cells for metastasis initiation. Cancer Cell. 22:571–584. 2012. View Article : Google Scholar : PubMed/NCBI
6	Cui G, Cai F, Ding Z and Gao L: MMP14 predicts a poor prognosis in patients with colorectal cancer. Hum Pathol. 83:36–42. 2019. View Article : Google Scholar : PubMed/NCBI
7	Ogata H, Goto S, Sato K, Fujibuchi W, Bono H and Kanehisa M: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 27:29–34. 1999. View Article : Google Scholar : PubMed/NCBI
8	Harris MA, Clark J, Ireland A, Lomax J, Ashburner M, Foulger R, Eilbeck K, Lewis S, Marshall B, Mungall C, et al: The Gene Ontology (GO) database and informatics resource. Nucleic Acids Res. 32((Database Issue)): D258–D261. 2004.PubMed/NCBI
9	Prescott JE, Osthus RC, Lee LA, Lewis BC, Shim H, Barrett JF, Guo Q, Hawkins AL, Griffin CA and Dang CV: A novel c-Myc-responsive gene, JPO1, participates in neoplastic transformation. J Biol Chem. 276:48276–48284. 2001. View Article : Google Scholar : PubMed/NCBI
10	Cho H, Lim BJ, Kang ES, Choi JS and Kim JH: Molecular characterization of a new ovarian cancer cell line, YDOV-151, established from mucinous cystadenocarcinoma. Tohoku J Exp Med. 218:129–139. 2009. View Article : Google Scholar : PubMed/NCBI
11	Lewis BC, Shim H, Li Q, Wu CS, Lee LA, Maity A and Dang CV: Identification of putative c-Myc-responsive genes: Characterization of rcl, a novel growth-related gene. Mol Cell Biol. 17:4967–4978. 1997. View Article : Google Scholar : PubMed/NCBI
12	Basu Baul TS, Longkumer I, Duthie A, Singh P, Koch B and Guedes da Silva MFC: Triphenylstannyl ((arylimino)methyl) benzoates with selective potency that induce G1 and G2/M cell cycle arrest and trigger apoptosis via ROS in human cervical cancer cells. Dalton Trans. 47:1993–2008. 2018. View Article : Google Scholar : PubMed/NCBI
13	Wang YC, Chang KC, Lin BW, Lee JC, Lai CH, Lin LJ, Yen Y, Lin CS, Yang SJ, Lin PC, et al: The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes. Exp Mol Med. 50:1–14. 2018. View Article : Google Scholar
14	Jiménez-P R, Martín-Cortázar C, Kourani O, Chiodo Y, Cordoba R, Domínguez-Franjo MP, Redondo JM, Iglesias T and Campanero MR: CDCA7 is a critical mediator of lymphomagenesis that selectively regulates anchorage-independent growth. Haematologica. 103:1669–1678. 2018. View Article : Google Scholar : PubMed/NCBI
15	Wang QL, Chen X, Zhang MH, Shen QH and Qin ZM: Identification of hub genes and pathways associated with retinoblastoma based on co-expression network analysis. Genet Mol Res. 14:16151–16161. 2015. View Article : Google Scholar : PubMed/NCBI
16	Albulescu R: Elevated cyclin B2 expression in invasive breast carcinoma is associated with unfavorable clinical outcome. BMC Cancer. 3:12013.
17	Osthus RC, Karim B, Prescott JE, Smith BD, McDevitt M, Huso DL and Dang CV: The Myc target gene JPO1/CDCA7 is frequently overexpressed in human tumors and has limited transforming activity in vivo. Cancer Res. 65:5620–5627. 2005. View Article : Google Scholar : PubMed/NCBI
18	Cheng C, Zhou Y, Li H, Xiong T, Li S, Bi Y, Kong P, Wang F, Cui H, Li Y, et al: Whole-genome sequencing reveals diverse models of structural variations in esophageal squamous cell carcinoma. Am J Hum Genet. 98:256–274. 2016. View Article : Google Scholar : PubMed/NCBI
19	Tate JG, Bamford S, Jubb HC, Sondka Z, Beare DM, Bindal N, Boutselakis H, Cole CG, Creatore C, Dawson E, et al: COSMIC: The catalogue of somatic mutations in cancer. Nucleic Acids Res. 47:D941–D947. 2019. View Article : Google Scholar : PubMed/NCBI
20	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
21	Bray F, Ferlay J, Soerjomataram I, Siegal RL, Torre LA and Jemal A: Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 68((6)): 394–424. 2018. View Article : Google Scholar : PubMed/NCBI
22	GBD 2015 Mortality and Causes of Death Collaborators, . Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980–2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet. 388((10053)): 1459–1544. 2016.PubMed/NCBI
23	Conover CA and Bale LK: Insulin-like growth factor I induction of c-myc expression in bovine fibroblasts can be blocked by antecedent insulin receptor activation. Exp Cell Res. 238:122–127. 1998. View Article : Google Scholar : PubMed/NCBI
24	Kitaura H, Shinshi M, Uchikoshi Y, Ono T, Iguchi-Ariga SM and Ariga H: Reciprocal regulation via protein-protein interaction between c-Myc and p21(cip1/waf1/sdi1) in DNA replication and transcription. J Biol Chem. 275:10477–10483. 2000. View Article : Google Scholar : PubMed/NCBI
25	Goto Y, Hayashi R, Muramatsu T, Ogawa H, Eguchi I, Oshida Y, Ohtani K and Yoshida K: JPO1/CDCA7, a novel transcription factor E2F1-induced protein, possesses intrinsic transcriptional regulator activity. Biochim Biophys Acta. 1759:60–68. 2006. View Article : Google Scholar : PubMed/NCBI
26	Zhang J, Sun Z, Han Y, Yao R, Yue L, Xu Y and Zhang J: Rnf2 knockdown reduces cell viability and promotes cell cycle arrest in gastric cancer cells. Oncol Lett. 13:3817–3822. 2017. View Article : Google Scholar : PubMed/NCBI
27	Lee J, Choi BY and Keum YS: Acetonitrile extract of Salvia miltiorrhizaRadix exhibits growth-inhibitory effects on prostate cancer cells through the induction of cell cycle arrest and apoptosis. Oncol Lett. 13:2921–2928. 2017. View Article : Google Scholar : PubMed/NCBI
28	Lu J, Chen X, Qu S, Yao B, Xu Y, Wu J, Jin Y and Ma C: Oridonin induces G₂/M cell cycle arrest and apoptosis via the PI3K/Akt signaling pathway in hormone-independent prostate cancer cells. Oncol Lett. 13:2838–2846. 2017. View Article : Google Scholar : PubMed/NCBI
29	Gill RM, Gabor TV, Couzens AL and Scheid MP: The MYC-associated protein CDCA7 is phosphorylated by AKT to regulate MYC-dependent apoptosis and transformation. Mol Cell Biol. 33:498–513. 2013. View Article : Google Scholar : PubMed/NCBI
30	Li D, Jiang X, Zhang X, Cao G, Wang D and Chen Z: Long noncoding RNA FGD5-AS1 promotes colorectal cancer cell proliferation, migration, and invasion through upregulating CDCA7 via sponging miR-302e. In Vitro Cell Dev Biol Anim. 55:577–585. 2019. View Article : Google Scholar : PubMed/NCBI