Open Access

miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells

  • Authors:
    • Yingjie Zhang
    • Lishu Zhou
    • Zhaoqiang Zhang
    • Fei Ren
    • Liangjiao Chen
    • Zedong Lan
  • View Affiliations

  • Published online on: May 4, 2020     https://doi.org/10.3892/mmr.2020.11110
  • Pages: 135-144
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The use of human bone marrow mesenchymal stem cells (hBMSCs) as a tissue engineering application for individuals affected by osteoporosis and other types of bone loss diseases has been well studied in recent years. The osteogenic differentiation of hBMSCs can be regulated by a number of cues. MicroRNAs (miRNAs/miRs) serve as the key regulators of various biological processes; however, to the best of our knowledge, no information exists with regards to the specific modulatory effects of miR‑10a‑5p on osteogenic differentiation of hBMSCs. The aim of the present study was to investigate the relationship between hBMSCs and miR‑10a‑5p and, ultimately, to determine how miR‑10a‑5p affects the osteogenic differentiation process of hBMSCs in vitro and in vivo. The hBMSCs used in the present study were transfected with mirVana™ miRNA inhibitors and mimics, and transfection efficiency was assessed by fluorescence microscopy and reverse transcription‑quantitative PCR (RT‑qPCR). Viability of hBMSCs following transfection was analyzed using a Cell Counting Kit‑8 assay. The mRNA expression levels of specific osteoblast markers, including alkaline phosphatase (ALP) and runt‑related transcription factor 2 (RUNX2) were measured using RT‑qPCR and western blot analysis. New bone formation was evaluated by Goldner's trichrome staining and micro‑CT analysis in vivo. No significant difference in cell viability was observed among the different groups 24 h post‑transfection. Overexpression of miR‑10a‑5p inhibited the expression of osteoblast makers in hBMSCs, whereas inhibition of miR‑10a‑5p upregulated the expression of ALP and RUNX2 in vitro. Furthermore, miR‑10a‑5p acted as a suppressor during the process of new bone formation in vivo. In conclusion, the findings of the present study suggested that miR‑10a‑5p served as a negative regulatory factor during osteoblast differentiation of hBMSCs and may be utilized in a treatment approach for bone repair in osteogenic‑related diseases.
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July-2020
Volume 22 Issue 1

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Y, Zhou L, Zhang Z, Ren F, Chen L and Lan Z: miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Mol Med Rep 22: 135-144, 2020.
APA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., & Lan, Z. (2020). miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Molecular Medicine Reports, 22, 135-144. https://doi.org/10.3892/mmr.2020.11110
MLA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22.1 (2020): 135-144.
Chicago
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22, no. 1 (2020): 135-144. https://doi.org/10.3892/mmr.2020.11110