Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

Liao,Xiaojie; Zhou,Zhuolin; Zhang,Xiaoliu

doi:10.3892/mmr.2020.11142

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

Authors:
- Xiaojie Liao
- Zhuolin Zhou
- Xiaoliu Zhang
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Affiliations: Department of Obstetrics, Jingmen No. 1 People's Hospital, Jingmen, Hubei 448000, P.R. China, Family Planning Ward, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530000, P.R. China
Published online on: May 12, 2020 https://doi.org/10.3892/mmr.2020.11142
Pages: 803-809
Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gestational diabetes mellitus (GDM) is a type of diabetes mellitus (DM) that occurs during pregnancy. The present study aimed to investigate the roles of microRNA (miR)‑195‑5p and enhancer of zeste homolog 2 (EZH2) in GDM, and their potential association. Human umbilical vein endothelial cells (HUVECs) were collected from healthy and GDM umbilical cords, and the endothelial properties were detected by flow cytometry. mRNA expression levels of miR‑195‑5p and EZH2, and EZH2 protein expression levels were detected by reverse transcription‑quantitative PCR (RT‑qPCR) and western blot analysis, respectively. Cell colony formation and flow cytometry were performed to determine cell proliferation and apoptosis. Furthermore, the target gene of miR‑195‑5p was predicted and assessed using a dual‑luciferase reporter assay. The levels of cell viability, proliferation and apoptosis following the overexpression of miR‑195‑5p, EZH2 or miR‑195‑5p + EZH2, were detected using Cell Counting Kit‑8, colony formation and flow cytometry assays, respectively. In addition, the mRNA expression levels of miR‑195‑59 and EZH2, and EZH2 protein expression levels following transfection with overexpression plasmids were detected using RT‑qPCR and western blot analysis, respectively. It was identified that high mRNA expression of miR‑195‑5p, and low EZH2 mRNA and protein expression levels decreased the level of cell proliferation and the high apoptotic rate of GDM‑HUVECs. In addition, miR‑195‑5p was predicted and identified to target EZH2, and miR‑195‑5p overexpression was identified to inhibit cell proliferation and promote apoptosis. However, it was demonstrated that upregulation of EZH2 could alleviate the inhibition of cell proliferation and the increased apoptotic rate induced by miR‑195‑5p overexpression. Therefore, the present results suggested that miR‑195‑5p may inhibit cell viability, proliferation and promote apoptosis by targeting EZH2 in GDM‑induced HUVECs.

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