Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology

Zhang,Yue; Zhang,Yue; Zhang,Yue; Zhang,Yue; Ou,Minglin; Ou,Minglin; Ou,Minglin; Ou,Minglin; Lin,Hua; Lin,Hua; Lin,Hua; Lin,Hua; Lai,Liusheng; Lai,Liusheng; Lai,Liusheng; Lai,Liusheng; Chen,Huaizhou; Chen,Huaizhou; Chen,Huaizhou; Chen,Huaizhou; Chen,Jiejing; Chen,Jiejing; Chen,Jiejing; Chen,Jiejing; Sui,Weiguo; Sui,Weiguo; Sui,Weiguo; Sui,Weiguo; Xue,Wen; Xue,Wen; Xue,Wen; Xue,Wen; Zhang,Ruohan; Zhang,Ruohan; Zhang,Ruohan; Zhang,Ruohan; Gan,Qing; Gan,Qing; Gan,Qing; Gan,Qing; Tang,Donge; Tang,Donge; Tang,Donge; Tang,Donge; Sun,Xuyong; Sun,Xuyong; Sun,Xuyong; Sun,Xuyong; Dong,Jianhui; Dong,Jianhui; Dong,Jianhui; Dong,Jianhui; Yan,Qiang; Yan,Qiang; Yan,Qiang; Yan,Qiang; Dai,Yong; Dai,Yong; Dai,Yong; Dai,Yong

doi:10.3892/mmr.2020.11299

Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology

Authors:
- Yue Zhang
- Minglin Ou
- Hua Lin
- Liusheng Lai
- Huaizhou Chen
- Jiejing Chen
- Weiguo Sui
- Wen Xue
- Ruohan Zhang
- Qing Gan
- Donge Tang
- Xuyong Sun
- Jianhui Dong
- Qiang Yan
- Yong Dai
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Affiliations: Department of Nephrology, Guilin No. 924 Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin Key Laboratory of Kidney Disease Research, Guilin, Guangxi 541002, P.R. China, Department of Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China, Department of Organ Transplantation, Institute of Transplant Medicine, No. 923 Hospital of People's Liberation Army, Guangxi Key Laboratory for Transplantation Medicine, Guangxi Transplantation Medicine Research Center of Engineering Technology, Nanning, Guangxi 530021, P.R. China
Published online on: July 6, 2020 https://doi.org/10.3892/mmr.2020.11299
Pages: 2329-2341
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transplantation is currently the best treatment for patients with end‑stage renal disease. However, acute rejection (AR) is the major source of failure in renal transplantation. The current best practice for the diagnosis of AR involves renal biopsy, but it is invasive, time‑consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve allograft function. The present study applied isobaric tags for relative and absolute quantitation (iTRAQ) mass spectrometry to analyze serum protein expression in patients with AR and healthy controls. Overall, 1,399 proteins were identified. Using a cut‑off of Q<0.05 and a fold change of >1.2 for the variation in expression, 109 proteins were identified to be differentially expressed between the AR and control groups, 72 of which were upregulated and 37 were downregulated. Several proteins, including properdin, keratin 1, lipoprotein(a) and vitamin D‑binding protein, may have roles in the pathogenesis of AR. The present study focused on iTRAQ‑based proteomic profiling of serum samples in AR. Insight from the present study may help advance the understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.

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