Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway

Yu,Mingyang; Wang,Yunguang; Zhang,Yao; Cui,Daping; Gu,Guishan; Zhao,Dewei

doi:10.3892/mmr.2020.11346

Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway

Authors:
- Mingyang Yu
- Yunguang Wang
- Yao Zhang
- Daping Cui
- Guishan Gu
- Dewei Zhao
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Affiliations: Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China, Laboratory of Molecular Biology, Institute of Nuclear‑Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China, Department of Orthopedics, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: July 16, 2020 https://doi.org/10.3892/mmr.2020.11346
Pages: 2741-2752
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gallium (Ga) ions have been widely utilized for biomedical applications; however, their role in osteoblast regulation is not completely understood. The aim of the present study was to investigate the potential effect of Ga ions on osteoinduction in two osteoblast cell lines and to explore the underlying mechanisms. Human hFOB1.19 and mouse MC3T3‑E1 osteoblasts were treated with Ga nitride (GaN) at different concentrations, and the degree of osteoinduction was assessed. Ga ion treatment was found to increase alkaline phosphatase activity and accelerate calcium nodule formation, as assessed using ALP activity assay and Alizarin red S staining. Moreover, upregulated expression levels of osteogenic proteins in osteoblasts were identified using western blotting and reverse transcription‑quantitative PCR. Western blotting was also performed to demonstrate that the biological action of Ga ions was closely associated with the transient receptor potential melastatin 7/Akt signaling pathway. Furthermore, it was found that Ga ions did not cause osteoblast apoptosis, as indicated via flow cytometry, but promoted osteoclast proliferation, migration or invasion. The present study investigated the potential role of Ga ions in regulating osteoinduction of osteoblasts, thereby providing a promising strategy for the treatment of osteoporosis.

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